T5327
Anti-TTP (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-G0/G1 switch regulatory protein 24, Anti-GOS24, Anti-Growth factor-inducible nuclear protein NUP475, Anti-NUP475, Anti-RNF162A, Anti-TIS11, Anti-Tristetraprolin, Anti-Zfp36, Zinc finger protein 36 homolog Protein
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg/mL using lysates of RAW264 cells induced with 10 ng/mL LPS for two hours
western blot: 0.5-1.0 μg/mL using lysates of RAW264 cells induced with 10 ng/mL LPS for two hours
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ZFP36(7538)
mouse ... Zfp36(22695)
rat ... Zfp36(79426)
Related Categories
General description
TTP belongs to a family of human ARE (AU-rich element found in 3′ UTR mRNA) binding protein TTP mRNA is widely distributed among tissue types, with a higher expression level in spleen, lymph nodes, and thymus.
Tristetraprolin (TTP), also known as zinc-finger protein 36 (ZFP36), is a RNA-binding protein, encoded by the gene mapped to human chromosome 19q13.2. TTP is ubiquitously expressed and is characterized with a highly homologous RNA binding domain (RBD) with two CCCH zinc fingers (ZFs).
Immunogen
synthetic peptide corresponding to amino acids 51-67 of human TTP, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.
Application
TTP (N-terminal) antibody produced in rabbit has been used in following studies:
- RNA EMSA supershift
- immunoblotting
- ribonucleoprotein immunoprecipitation (RNP-IP)
Biochem/physiol Actions
In activated monocytes and T lymphocytes, it regulates the expression of tumor necrosis factor a (TNF- α) by binding to a conserved AU rich element within the 3′ UTR of TNF-α mRNA. TTP is a phosphoprotein reported to be a substrate for kinases like MAPK activated protein kinase 2 (MAPKAPK2). The p38 signal transduction pathway is required for the expression of TTP protein and mRNA.
Tristetraprolin (TTP) plays a key role in maintaining the stability of certain adenosine/uridine AU-rich element (ARE) mRNAs, by removing of the poly(A) tail and enhancing mRNA turnover. The encoded protein participates in mRNA binding and destabilization.
Target description
TTP (N-terminal) (also known as Tristetraproline, Zfp-36, TIS11A, and Growth factor-inducible nuclear protein NUP475) is an RNA-binding protein that suppresses inflammation by accelerating the degradation of cytokine mRNA. TTP belongs to a family of human
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Tristetraprolin suppresses the EMT through the down-regulation of Twist1 and Snail1 in cancer cells.
Nal Ae Yoon et al.
Oncotarget, 7(8), 8931-8943 (2016-02-04)
Inhibition of epithelial-mesenchymal transition (EMT)-inducing transcription factors Twist and Snail prevents tumor metastasis but enhances metastatic growth. Here, we report an unexpected role of a tumor suppressor tristetraprolin (TTP) in inhibiting Twist and Snail without enhancing cellular proliferation. TTP bound
The tandem CCCH zinc finger protein tristetraprolin and its relevance to cytokine mRNA turnover and arthritis
Carrick DM, et al.
Arthritis Research & Therapy, 6(6), 248-248 (2004)
Yingqing Chen et al.
Oxidative medicine and cellular longevity, 2017, 9427583-9427583 (2017-06-14)
Pterostilbene, a dimethyl ester analog of resveratrol, has anti-inflammatory and antioxidative effects and alters cell proliferation. Tristetraprolin (TTP) promotes the degradation of proinflammatory mediators via binding to adenosine and uridine- (AU-) rich elements (ARE) located in the 3'-untranslated regions of
Qinghong Wang et al.
Nature communications, 8(1), 867-867 (2017-10-13)
IFN-γ-producing cytotoxic T lymphocytes are essential for host defense against viral infection and cancer. Here we show that the RNA-binding tristetraprolin, encoded by Zfp36, is needed for CD8+ T-cell production of IFN-γ in vivo. When activated in vitro, however, IFN-γ
ELAVL1 Modulates Transcriptome-wide miRNA Binding in Murine Macrophages
Lu YC
Cell Reports, 9, 2330-2343 (2014)
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