T4904
Tris-Glycine Buffer 10× Concentrate
Synonym(s):
running buffer
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About This Item
UNSPSC Code:
41105323
EC Number:
232-650-8
NACRES:
NA.25
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Application
Tris-Glycine buffer 10× concentrate has been used as a transfer buffer for Western blotting.
Biochem/physiol Actions
For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.
Other Notes
0.25 M Tris, 1.92 M glycine, pH approx. 8.3
Contains no methanol.
Contains no methanol.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Immunoblotting and dot blotting.
D I Stott
Journal of immunological methods, 119(2), 153-187 (1989-05-12)
Multiple tandem epitope tagging for enhanced detection of protein expressed in mammalian cells.
Zhang L, et al.
Molecular Biotechnology, 19, 313-321 (2001)
H Towbin et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(9), 4350-4354 (1979-09-01)
A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was
Protocols
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
带有用于不同印迹过程工作流程步骤的Western blot实验方法，描述了蛋白从SDS聚丙烯酰胺凝胶(SDS-PAGE)到硝酸纤维素膜(NC膜)的电泳转移。也称为免疫印迹实验方法。
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