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T4661

Sigma-Aldrich

Trizma® base

≥99.9% (titration), crystalline

Synonym(s):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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5 G
CN¥482.77
25 G
CN¥531.99
100 G
CN¥995.77

About This Item

Linear Formula:
NH2C(CH2OH)3
CAS Number:
77-86-1
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
201-064-4
MDL number:
MFCD00004679
UNSPSC Code:
12352104
PubChem Substance ID:
24900061
NACRES:
NA.25

CN¥482.77

Estimated to ship onApril 07, 2025Details

Request a Bulk Order

description

aminopeptidase substrate

Quality Level

400

Assay

≥99.9% (titration)

form

crystalline

loss

≤5% (loss on drying, 110 °C)

color

white

pH

10.0-11.5

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

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material

polypropylene tube (for collection), styrene-butadiene housing, regenerated cellulose membrane

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

product line

Amicon®

product line

Amicon®

product line

Amicon®

product line

Amicon®

parameter

2 mL sample volume

parameter

2 mL sample volume

parameter

2 mL sample volume

parameter

2 mL sample volume

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[1] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[2] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.[3]

Application

Tris base has been used:
  • As a component of lysis buffer for cell disruption[4][5]
  • In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)[6][5]
  • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC[7]
  • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols[8]

Packaging

Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.

Analysis Note

Not pyrogen tested. Certificates of analysis are available with lot specific information.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000430883
    0000430883
    0000419003
    0000419021
    0000419022

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells.
    Caglayan M, et al.
    Bio-protocol, 5(11) (2015)
    Accumulated lipids rather than the rigid cell walls impede the extraction of genetic materials for effective colony PCRs in Chlorella vulgaris.
    Tear CJY, et al.
    Microbial cell factories, 12(1), 106-106 (2013)
    Genome-wide mapping of embedded ribonucleotides and other noncanonical nucleotides using emRiboSeq and EndoSeq
    Ding J, et al.
    Nature Protocols, 10(9), 1433-1433 (2015)
    Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs). High-performance ionexchange chromatographic analysis
    Lesignoli F, et al.
    Journal of Chromatography A, 922(1-2), 177-185 (2001)
    Capillary electrochromatographic evaluation of vitamin active oil constituents: tocopherols and tocotrienols.
    Abidi SL and Rennick KA
    Journal of Chromatography A, 913(1-2), 379-386 (2001)
    View All Related Papers

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