T4202
TRKA (440-end), active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
DKFZp781l14186, MTC, NTRK1, TRK1, p140-TrKA
biological source
human
Quality Level
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
Assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
19-25 nmol/min·mg
mol wt
~66 kDa
technique(s)
activity assay: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NTRK1(4914)
Application
TRKA (440-end), active, GST tagged human was used to study functional NGF signal transduction.
Biochem/physiol Actions
TRKA (440-end), active, GST tagged human is a 140 kD membrane-spanning protein tyrosine kinase that belongs to proto-oncogene family. TRKA facilitates the regulation of cell differentiation and survival via activating SHC1 and FRS2 which in turn activates a GRB2-Ras-MAPK cascade. It also plays a pivotal role in controlling NF-κ-B activation as well as transcription of genes involved in cell survival via PLCG1.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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F Iannone et al.
Rheumatology (Oxford, England), 41(12), 1413-1418 (2002-12-07)
We aimed to investigate the expression of nerve growth factor (NGF) and high affinity NGF receptor (p140 TrkA) on chondrocytes from human healthy and osteoarthritic cartilage. We recruited 12 patients with osteoarthritis (OA) undergoing surgical knee replacement. Articular cartilage was
F Boissière et al.
Molecular and chemical neuropathology, 28(1-3), 219-223 (1996-05-01)
The most consistent neuropathological finding in Alzheimer disease (AD) is the loss of cholinergic neurons of the nucleus basalis of Meynert (NbM). Using immunohistochemistry, we have previously shown that cholinergic neurons located in the ventral striatum were affected, whereas those
R M Stephens et al.
Neuron, 12(3), 691-705 (1994-03-01)
In response to NGF, the Trk receptor tyrosine kinase forms a complex with SHC, a protein that couples receptor tyrosine kinases to p21ras. Complex formation between Trk and SHC, SHC tyrosine phosphorylation, and association of SHC with Grb2 were mediated
Ilaria Caon et al.
Cancers, 13(5) (2021-04-04)
Interaction between cancer cells and their microenvironment is central in defining the fate of cancer development. Tumour cells secrete signals (cytokines, chemokines, growth factors) that modify the surrounding area, while the niche supplies structures and activities necessary for tumour maintenance
D R Kaplan et al.
Science (New York, N.Y.), 252(5005), 554-558 (1991-04-26)
The trk proto-oncogene encodes a 140-kilodalton, membrane-spanning protein tyrosine kinase (p140prototrk) that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of p140prototrk in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium
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