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T3195

Sigma-Aldrich

Anti-γ-Tubulin (DQ-19) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-CDCBM4, Anti-GCP-1, Anti-TUBG, Anti-TUBGCP1

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About This Item

MDL number:
MFCD00677366
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

chicken, human

technique(s)

indirect immunofluorescence: 1:500 using methanol-acetone fixed, cultured chicken fibroblasts.
western blot: 1:1,000 using whole cell extract of human epidermal carcinoma A431 cell line.

UniProt accession no.

P23258

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBG1(7283) , TUBG2(27175)

General description

Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells. It is a member of the tubulin superfamily of proteins which includes α- and β- tubulin and the newly discovered centrosomal-associated proteins, δ- and ε- tubulin. γ-Tubulin is a part of large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule. Its abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares 28-32% identity with α-tubulin from various organisms, 32-36% identity with β-tubulins and 29-30% identity with δ- and ε tubulins. Some regions including those thought to be involved in GTP) binding are highly conserved among α-, β-, γ-, δ-, and ε-tubulins.

Immunogen

synthetic peptide corresponding to the C-terminus of human γ-tubulin (amino acids 433-451 with N-terminally added lysine), conjugated to KLH.

Application

Anti-γ-Tubulin (DQ-19) antibody produced in rabbit has been used in:
  • western blotting
  • immunocytochemistry
  • immunofluorescence

Biochem/physiol Actions

The proper organization of microtubules is essential for cell division and chromosome segregation, directed cell movement, interphase cytoplasmic organization, and other cytoskeletal functions. γ-Tubulin has an essential role in microtubule nucleation by the centrosomes. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-TuRC (γ-tubulin ring complex) binds the microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Nihal Kaplan et al.
The ocular surface, 21, 193-205 (2021-06-14)
To understand the relationship between ciliogenesis and autophagy in the corneal epithelium. siRNAs for EphA2 or PLD1 were used to inhibit protein expression in vitro. Morpholino-anti-EphA2 was used to knockdown EphA2 in Xenopus skin. An EphA2 knockout mouse was used
The centrosomal kinase Aurora-A/STK15 interacts with a putative tumor suppressor NM23-H1
Du J and Hannon GJ
Nucleic Acids Research, 30(24), 5465-5475 (2002)
The elongation of primary cilia via the acetylation of a-tubulin by the treatment with lithium chloride in human fibroblast KD cells.
Nakakura T
Medical Molecular Morphology, 48(1), 44-53 (2015)
Microtubule-organizing centres: a re-evaluation
Luders J and Stearns T
Nature Reviews in Molecular and Cell Biology, 8(2), 161-161 (2007)
Jianqiu Zou et al.
Biology open, 2(10), 1022-1031 (2013-10-30)
DNA damage response (DDR) and the centrosome cycle are two of the most critical processes for maintaining a stable genome in animals. Sporadic evidence suggests a connection between these two processes. Here, we report our findings that six Fanconi Anemia
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Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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