T3194
Tetanus toxin
from Clostridium tetani, cleaves synaptobrevin (VAMP-2)
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About This Item
product name
Tetanus toxin from Clostridium tetani,
mol wt
150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)
Quality Level
solubility
H2O: soluble
storage temp.
2-8°C
Gene Information
Clostridium tetani E88 ... tetX(1061100)
General description
Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein, produced by Clostridium tetani. This protein consists of a light and a heavy chain.
Application
Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway. It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening.
Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells1.
Biochem/physiol Actions
Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.
Tetanus toxin can cause spastic paralysis.
Preparation Note
Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.
Reconstitution
Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.
Analysis Note
Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 1 Oral - Acute Tox. 2 Inhalation - STOT SE 1
Target Organs
Central nervous system
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
新产品
Certificates of Analysis (COA)
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Tetanus
Hunter's Tropical Medicine and Emerging Infectious Disease, 508-510 (2013)
Felix Horns et al.
Cell reports, 30(3), 905-913 (2020-01-23)
Antibody memory protects humans from many diseases. Protective antibody memory responses require activation of transcriptional programs, cell proliferation, and production of antigen-specific antibodies, but how these aspects of the response are coordinated is poorly understood. We profile the molecular and
MODELS vert The Tetanus Toxin Model of Temporal Lobe Epilepsy
Endocrine regulations, 804-807 (2009)
Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of alpha-synuclein via exosome
Yang Y, et al.
PLoS ONE, 12(7), e0180892-e0180892 (2017)
Rodolfo M Iglesias et al.
Neurochemical research, 37(6), 1355-1363 (2012-02-24)
Pannexin1 (Panx1), a protein related to the gap junction proteins of invertebrates, forms nonjunctional channels that open upon depolarization and in response to mechanical stretch and purinergic receptor stimulation. Importantly, ATP can be released through Panx1 channels, providing a possible
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