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T2949

Sigma-Aldrich

Anti-mTOR antibody produced in rabbit

1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-FKBP-12 rapamycin associated protein, Anti-FRAP, Anti-RAFT, Anti-SEP

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About This Item

MDL number:
MFCD02263996
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 289 kDa

species reactivity

rat, human, mouse

concentration

1.0-1.5 mg/mL

technique(s)

immunoprecipitation (IP): 1.0-1.5 μg using 300 μg RIPA extract of human transformed kidney HEK 293T cells
microarray: suitable
western blot: 1:2,000 using whole extracts of rat brain tissue
western blot: 1:500 using mouse brain tissue

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FRAP1(2475)
mouse ... Frap1(56717)
rat ... Frap1(56718)

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General description

mTOR is an evolutionary conserved serine/threonine kinase that belongs to the phosphoinositol kinase-related kinase (PIKK) family. This kinase regulates cell growth by initiation of translation in response to nutrients such as amino acids (primarily leucine), growth factors, mitogens and insulin. Translational initiation is mediated by mTOR through S6K1 activation and 4E-BP1 inhibition. mTOR may also modulate several cellular functions such as actin organization, secretion, membrane trafficking, protein degradation, protein kinase C signaling, tRNA synthesis and ribosome biogenesis. Anti-mTOR antibody is specific for human, mouse and rat mTOR (289 kDa).

Specificity

Anti-mTOR (FRAP) is produced using a peptide corresponding to amino acids 2433-2450 of human mTOR. The corresponding sequence is identical in mouse and differs by one amino acid in rat. The antibody specifically recognizes human, mouse and rat mTOR (289 kDa) and is suitable for immunoblotting, immunoprecipitation and immunofluorescence.

Immunogen

The corresponding sequence is identical in mouse and differs by one amino acid in rat.
synthetic peptide corresponding to amino acid residues 2433-2450 of human mTOR.

Application

Anti-mTOR antibody is suitable for use in Western blot (1:1,000 using myotube cell lysates of rainbow trout cultures). The antibody can also be used in indirect immunofluorescence, immunoprecipitation, and microarray.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

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Flash Point(F)

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Flash Point(C)

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Regulatory Information

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Daisuke Nanba et al.
EMBO reports, 24(6), e55439-e55439 (2023-05-04)
Adult autologous human epidermal stem cells can be extensively expanded ex vivo for cell and gene therapy. Identifying the mechanisms involved in stem cell maintenance and defining culture conditions to maintain stemness is critical, because an inadequate environment can result
Sara Comesaña et al.
Frontiers in physiology, 10, 1206-1206 (2019-10-18)
We hypothesize that the presence in fish brain of a ketone body (KB) like β-hydroxybutyrate (BHB) alters energy homeostasis through effects on food intake and peripheral energy metabolism. Using rainbow trout (Oncorhynchus mykiss) as a model, we intracerebroventricularly (ICV) administered
F Lavajoo et al.
Scientific reports, 10(1), 184-184 (2020-01-15)
The tolerance of fish to fasting offers a model to study the regulatory mechanisms and changes produced when feeding is restored. Gilthead sea bream juveniles were exposed to a 21-days fasting period followed by 2 h to 7-days refeeding. Fasting provoked
mTOR signaling regulates myotube hypertrophy by modulating protein synthesis, rDNA transcription, and chromatin remodeling
Von Wolden F, et al.
The American Journal of Physiology, 311(4) (2016)
Sara Comesaña et al.
Frontiers in physiology, 9, 1209-1209 (2018-09-14)
To continue gathering knowledge on the central regulation of food intake in response to amino acids in teleost fish, using as a model rainbow trout (Oncorhynchus mykiss), we evaluated in a first experiment the feeding attractiveness of L-leucine, L-valine, and
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