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Merck
CN

T2145

Tyrode’s Salts

Powder, without sodium bicarbonate, suitable for cell culture

Synonym(s):

Tyrode′s Buffer, Tyrode′s Solution, Tyrode′s buffer

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
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Product Name

Tyrode′s Salts, Without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

General description

Tyrode′s Salts play an important role in tissue culture. A salt solution′s basic function is to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Application

Tyrode′s Salts has been used:
  • for seeding cardiomyocytes culture for optical based phenotyping and imaging studies
  • to incubate 3T3-L1 pre-adipocytes in calcium intracellular assays
  • as a medium for the isolation and culturing of myofiber extensor digitorum longus (EDL) muscle

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.
Supplement with 1.0 g/L sodium bicarbonate.


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Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基及盐混合物极具吸湿性,应注意防潮。


Theodora Pavlidou et al.
Stem cells international, 2019, 5980465-5980465 (2019-06-30)
The regeneration of the muscle tissue relies on the capacity of the satellite stem cell (SC) population to exit quiescence, divide asymmetrically, proliferate, and differentiate. In age-related muscle atrophy (sarcopenia) and several dystrophies, regeneration cannot compensate for the loss of
Yu-Fen Chang et al.
PloS one, 12(4), e0174181-e0174181 (2017-04-06)
Identification of drug induced electrical instability of the heart curtails development, and introduction, of potentially proarrhythmic drugs. This problem usually requires complimentary contact based approaches such as patch-clamp electrophysiology combined with field stimulation electrodes to observe and control the cell.
Anna Marianne Weijler et al.
Frontiers in immunology, 11, 586856-586856 (2020-12-29)
Humoral immunity is a major barrier limiting long-term outcome after organ transplantation. Especially, the production of antibodies directed against donor HLA/MHC antigens (i.e. donor-specific antibodies (DSA)) leading to antibody-mediated rejection (ABMR) is considered to be a major factor negatively affecting



Global Trade Item Number

SKUGTIN
T2145-10L04061837341601
T2145-100L04061837474408
T2145-10X1L04061837341618
T2145-25K04061832957982