T1895
Thymidine
powder, BioReagent, suitable for cell culture
Synonym(s):
1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, 1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine deoxyriboside, dT
About This Item
biological source
synthetic (organic)
Quality Level
product line
BioReagent
Assay
≥99%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
186-188 °C (lit.)
solubility
water: 50 mg/mL, clear, colorless
SMILES string
CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O
InChI
1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1
InChI key
IQFYYKKMVGJFEH-XLPZGREQSA-N
Gene Information
human ... DTYMK(1841)
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General description
Application
- as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts
- as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies
- to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells
Biochem/physiol Actions
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How can I solubilize Product T1895, Thymidine?
This product is soluble in water (50 mg/mL), yielding a clear, colorless solution with heating. For aqueous concentrations higher than 50 mg/mL, solubility can be enhanced with the addition of a small amount of NaOH solution. Add the base drop wise with slow stirring. Thymidine is also soluble in methanol, hot alcohol, hot acetone, hot ethyl acetate, pyridine, glacial acetic acid, and is sparingly soluble in hot chloroform.
What concentration of Product T1895, Thymidine, is used for testing this product for cell culture applications?
The tested working concentration for cell culture for Thymidine is 8 mg/L.
How can I sterilize a solution of Product T1895, Thymidine?
Solutions can be sterile filtered using 0.2 μm filters (low protein binding). Do not autoclave solutions.
How long can I store media supplemented with Product T1895, Thymidine?
Thymidine is a component of Nutrient Mixture F-12 at 0.73mg/L. This media has an expiration date of 18 months from date of formulation. You will need to determine if this is appropriate for your application.
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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