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T1827

Sigma-Aldrich

Anti -TBP antibody, Mouse monoclonal

clone 58C9, purified from hybridoma cell culture

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Synonym(s):
Anti-GTF2D, Anti-GTF2DI, Anti-SCA17, Anti-TATA box binding protein, Anti-TFIID
MDL number:
MFCD01322691
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

58C9

mol wt

antigen ~42 kDa

species reactivity

Sf9 cell line, human, yeast, Drosophila melanogaster

technique(s)

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using nuclear extracts of D.Mel cells

isotype

IgG2b

UniProt accession no.

P20226

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Drosophila melanogaster ... Tbp(37476)
human ... TBP(6908)

General description

Monoclonal Anti-TBP (mouse IgG2b isotype) is derived from the hybridoma 58C9 produced by the fusion of mouse myeloma cells and splenocytes from Swiss Webster mice immunized with Drosophila TFIID complex. Transcription factor IID (TFIID) complex contains the TATA-binding protein (TBP) and over a dozen of TBP-associated factors (TAFs).
TBP (TATA-binding protein) is encoded by the gene mapped to human chromosome 6q27. The encoded protein is a key constituent of RNA polymerase II transcription apparatus in eukaryotic cells.

Immunogen

Drosophila TFIID complex.

Application

Monoclonal Anti-TBP antibody produced in mouse has been used in:
  • immunoblotting
  • co-immunoprecipitation
  • immunohistochemistry

Biochem/physiol Actions

TBP facilitates promoter recognition with the help of the sequence-specific binding of the TATA element found in many promoters.
The TBP (TATA-binding protein) gene encodes a subunit of TFIIIB (transcription factor IIIB) complex and recognizes the TATA box in the DNA. Thus, it plays an important role in RNA polymerase II transcription machinery. TBP is known to induce stress granule formation in hepatitis B viral infection. Mutation in the gene is associated with the development of spinocerebellar ataxia 17 (SCA17).

Target description

TBP encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of tr

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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  5. Which light chain is used in Product T1827, Monoclonal Anti-TBP antibody, clone 58C9?

    The light chain for Product T1827, Monoclonal Anti-TBP antibody, clone 58C9 is a lambda light chain.

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Enhanced Human Decidual Cell-Expressed FKBP51 May Promote Labor-Related Functional Progesterone Withdrawal
Schatz F, et al.
The American Journal of Pathology, 185(9), 2402-2411 (2015)
Doubly Spliced RNA of Hepatitis B Virus Suppresses Viral Transcription via TATA-Binding Protein and Induces Stress Granule Assembly.
Tsai KN
Journal of Virology, 89, 11406-11419 (2015)
K Nakamura et al.
Human molecular genetics, 10(14), 1441-1448 (2001-07-13)
Genetic etiologies of at least 20% of autosomal dominant cerebellar ataxias (ADCAs) have yet to be clarified. We identified a novel spinocerebellar ataxia (SCA) form in four Japanese pedigrees which is caused by an abnormal CAG expansion in the TATA-binding
Taspase1 processing alters TFIIA cofactor properties in the regulation of TFIID.
Malecova B
Transcription, 6, 21-32 (2015)
The Pathogenic Role of Low Range Repeats in SCA17.
Shin JH
PLoS ONE, 10 (2015)
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