T1788
Tyrode′s Solution, Acidic
liquid, sterile-filtered, suitable for mouse embryo cell culture
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UNSPSC Code:
12352207
NACRES:
NA.75
Recommended Products
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | embryo: suitable
impurities
endotoxin, tested
pH
2.5±0.3
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
Tyrode′s salt solution is used for diluting protein components of media of natural origin. The current role of a balanced salt solution in cell culture is multifaceted. It serves as an irrigating, transporting, and diluting agent while maintaining intra- and extracellular osmotic balance and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It also provides the essential energy for cell metabolism and a buffering system to maintain the medium within the optimal pH range (7.2-7.6).
Application
Tyrode′s Solution, Acidic has been used for the removal of zona pellucida from oocytes/embryos.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Sara Carvalhal et al.
Molecular biology of the cell, 28(19), 2470-2478 (2017-08-05)
Asymmetric cell divisions depend on the precise placement of the spindle apparatus. In mammalian oocytes, spindles assemble close to the cell's center, but chromosome segregation takes place at the cell periphery where half of the chromosomes are expelled into small
Liyan Sui et al.
Journal of cellular physiology, 235(10), 7030-7042 (2020-02-06)
Histone deacetylase 6 (HDAC6) participates in mouse oocyte maturation by deacetylating α-tubulin. However, how HDAC6 expression is regulated in oocytes remains unknown. In the present study, we discovered that mouse oocytes had a high level of HDAC6 expression and a
Maryam Salimi et al.
Reports of biochemistry & molecular biology, 9(3), 357-365 (2021-03-03)
Currently, the efficient production of chimeric mice and their survival are still challenging. Recent researches have indicated that preimplantation embryo culture media and manipulation lead to abnormal methylation of histone in the H19/Igf2 promotor region and consequently alter their gene
Qianhua Xu et al.
Nature genetics, 51(5), 844-856 (2019-05-02)
The oocyte epigenome plays critical roles in mammalian gametogenesis and embryogenesis. Yet, how it is established remains elusive. Here, we report that histone-lysine N-methyltransferase SETD2, an H3K36me3 methyltransferase, is a crucial regulator of the mouse oocyte epigenome. Deficiency in Setd2
Guojing Chang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(7), 2760-2770 (2017-03-16)
During pregnancy, placental trophoblasts at the feto-maternal interface produce a broad repertoire of microRNA (miRNA) species. These species include miRNA from the primate-specific chromosome 19 miRNA cluster (C19MC), which is expressed nearly exclusively in the placenta. Trafficking of these miRNAs
Articles
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
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