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Key Documents

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T1283

Sigma-Aldrich

holo-Transferrin bovine

Iron-saturated, BioReagent, suitable for cell culture

Synonym(s):

Siderophilin, Siderophilin, iron-saturated

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About This Item

CAS Number:
11096-37-0
EC Number:
234-318-8
MDL number:
MFCD00131337
UNSPSC Code:
12352202
NACRES:
NA.75
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product line

BioReagent

Assay

97-100% (agarose gel electrophoresis)

form

powder

concentration

~25 mM

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

H2O: 50 mg/mL

UniProt accession no.

Q29443

shipped in

ambient

storage temp.

2-8°C

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全转铁蛋白 牛 Iron-saturated, BioReagent, suitable for cell culture

T1283

全转铁蛋白 牛

脱铁-转铁蛋白 牛 BioReagent, suitable for cell culture, ≥98%

T1428

脱铁-转铁蛋白 牛

转铁蛋白 人 powder, BioReagent, suitable for cell culture

T8158

转铁蛋白 人

脱铁-转铁蛋白 牛 ≥98%

T0178

脱铁-转铁蛋白 牛

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

solubility

H2O: 50 mg/mL

solubility

H2O: 10 mg/mL

solubility

H2O: 50 mg/mL

solubility

H2O: 20 mg/mL

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Bovine holo-transferrin can be used with a wide range of cells types. Bovine holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.

Analysis Note

Purity by agarose gel electrophoresis.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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    Lot/Batch Number
    0000318340
    0000318320
    0000318321
    0000316202
    0000316190

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    Han Hu et al.
    Animal science journal = Nihon chikusan Gakkaiho, 87(1), 84-91 (2015-10-16)
    In this study, bovine mammary epithelial cells were used to study stress responses after cells were exposed to 42°C for 0.5, 1, 3, 5, 8 or 12 h, and 38°C as control. The transcription of the genes (HSP27, HSP70 and
    William Schachterle et al.
    Nature communications, 8, 13963-13963 (2017-01-17)
    Transplanting vascular endothelial cells (ECs) to support metabolism and express regenerative paracrine factors is a strategy to treat vasculopathies and to promote tissue regeneration. However, transplantation strategies have been challenging to develop, because ECs are difficult to culture and little
    Wenting Dai et al.
    The British journal of nutrition, 123(5), 489-498 (2019-11-13)
    Despite the well-characterised mechanisms of amino acids (AA) regulation of milk protein synthesis in mammary glands (MG), the underlying specific AA regulatory machinery in bovine MG remains further elucidated. As methionine (Met) is one of the most important essential and
    Samuel G Rodriques et al.
    Nature biotechnology, 39(3), 320-325 (2020-10-21)
    Current approaches to single-cell RNA sequencing (RNA-seq) provide only limited information about the dynamics of gene expression. Here we present RNA timestamps, a method for inferring the age of individual RNAs in RNA-seq data by exploiting RNA editing. To introduce
    Guihua Du et al.
    Development (Cambridge, England), 147(8) (2020-03-20)
    Continual spermatogenesis relies on the actions of an undifferentiated spermatogonial population that is composed of stem cells and progenitors. Here, using mouse models, we explored the role of RNA-binding proteins (RBPs) in regulation of the biological activities of this population.
    View All Related Papers

    Articles

    Transferrin in Cell Culture

    Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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