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Synonym(s):
10X TTS Buffer, 10X Tris Tricine SDS running buffer, Tris Tricine SDS transfer buffer
UNSPSC Code:
41105319
NACRES:
NA.25
Recommended Products
sterility
0.2 μm filtered
Quality Level
form
liquid
pH
8.05-8.30 (25 °C in water, high purity, 1:10)
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Related Categories
General description
Tris-Tricine-SDS (TTS) running buffer is the cathode (upper reservoir) buffer for SDS−polyacrylamide gel electrophoresis of proteins using the Schagger and von Jagow method. The Schagger and von Jagow method is designed for the separation of small molecular weight proteins. It differs from the Laemmli method in that the glycine is replaced with tricine and the gel contains 1M Tris-HCl, pH 8.45 instead of 0.375 M Tris-HCl, pH 8.9. Dilution of the 10X TTS buffer produces a 1X cathode buffer containing 100 mM Tris, 100 mM tricine and 0.1% SDS. Recommended running conditions is 125 volts.
Application
Tris-Tricine-SDS Buffer 10× Concentrate is suitable for use as a running buffer for polyacrylamide based separation of bronchoalveolar lavage fluid (BALF).
Other Notes
1M Tris, 1M Tricine, 1% (w/v) SDS, pH approx. 8.2.
Analysis Note
Tested for use as a cathode buffer for Tris-Tricine gel electrophoresis to separate low molecular weight proteins.
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Nonviral gene delivery to the lung with copolymer-protected and transferrin-modified polyethylenimine
Rudolph C, et al.
Biochim. Biophys. Acta Gen. Subj., 1573(1), 75-83 (2002)
H Schägger et al.
Analytical biochemistry, 166(2), 368-379 (1987-11-01)
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations
Bonnie J Berry et al.
ACS chemical neuroscience, 9(7), 1693-1701 (2018-05-11)
Alzheimer's disease (AD) is characterized by slow, progressive neurodegeneration leading to severe neurological impairment, but current drug development efforts are limited by the lack of robust, human-based disease models. Amyloid-β (Aβ) is known to play an integral role in AD
Rachel E Gerver et al.
Analytical chemistry, 86(21), 10625-10632 (2014-10-01)
We describe a microfluidic Western blot assay (μWestern) using a Tris tricine discontinuous buffer system suitable for analyses of a wide molecular mass range (6.5-116 kDa). The Tris tricine μWestern is completed in an enclosed, straight glass microfluidic channel housing
Wataru Ohwada et al.
Biochimica et biophysica acta. Molecular basis of disease, 1866(10), 165851-165851 (2020-06-02)
ERK and Akt have been shown to regulate cell sensitivity to death-inducing stress by phosphorylating GSK-3β, a major modulator of the threshold for mitochondrial permeability transition. Here we examined intra-mitochondrial localization of the pro-survival kinases and their regulation by phosphatases.
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