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T0910

Thioredoxin from Escherichia coli

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

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About This Item

CAS Number:
52500-60-4
UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
MFCD00132423

Key Documents

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Product Name

Thioredoxin from Escherichia coli, recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

biological source

Escherichia coli

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

specific activity

≥3 units/mg protein

mol wt

11.7 kDa

technique(s)

activity assay: suitable

solubility

water: 1.90-2.10 mg/mL, clear, colorless

Protein ID accession no.

M26133

UniProt accession no.

P0AA25

application(s)

cell analysis

storage temp.

−20°C

Quality Level

200

Gene Information

Escherichia coli K12 ... trxA(948289)

Application

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay. It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease.

Biochem/physiol Actions

Mediates the reduction of disulfide bonds in proteins.
Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication. Additionally, it plays a role in regulating cell growth and apoptosis.

General description

Research area: Cell Signaling

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.

Other Notes

GenBank® Accession Number M26133.
One unit will cause a ΔA650 of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000354982
0000354982
0000222615
0000222615
0000137429

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Apoptosis signal-regulating kinase 1-thioredoxin complex dissociation by capsaicin causes pancreatic tumor growth suppression by inducing apoptosis
Pramanik KC, et al.
Antioxidants & Redox Signaling, 1417-1432 (2012)
Thioredoxin from Escherichia coli as a Role Model of Molecular Recognition, Folding, Dynamics and Function.
Vazquez DS
Protein and peptide letters, 22(9), 801-815 (2015)
Cell Signaling with Extracellular Thioredoxin and Thioredoxin-Like Proteins: Insight into Their Mechanisms of Action
Leveillard T and Ait-Ali N
Oxidative Medicine and Cellular Longevity (2017)
Thioredoxin Delays Photoreceptor Degeneration, Oxidative and Inflammation Alterations in Retinitis Pigmentosa
Hernandez RG, et al.
Frontiers in Pharmacology (2020)
Apoptosis signal-regulating kinase 1-thioredoxin complex dissociation by capsaicin causes pancreatic tumor growth suppression by inducing apoptosis
Pramanik KC, et al.
Journal of Separation Science, 17(10), 1417-1432 (2012)
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