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Safety Information

T0665

Sigma-Aldrich

holo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥97%

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Synonym(s):
Siderophilin, iron-saturated
CAS Number:
11096-37-0
EC Number:
234-318-8
MDL number:
MFCD00131337
UNSPSC Code:
12352202
NACRES:
NA.75

biological source

human plasma

Quality Level

200

description

Iron-saturated

product line

BioReagent

Assay

≥97%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

HIV and HBsAg, none detected
 endotoxin, tested

solubility

H2O: 50 mg/mL

UniProt accession no.

P02787

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... TF(7018)

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Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.
holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.

Biochem/physiol Actions

Provides iron to cells in a physiologically stable and safe form.

Preparation Note

Heat treated at 60 °C (minimum) for a minimum of 10 hours.

Analysis Note

Iron content by ICP reported on a lot-specific basis.
Purity by agarose gel electrophoresis.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 3

Regulatory Information

常规特殊物品

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the carbohydrate content of human transferrin?

    We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.

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