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T0647

Sigma-Aldrich

TAPS sodium salt

≥99% (titration)

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Synonym(s):
3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid sodium salt, TAPS NA, N-tris(Hydroxymethyl)methyl-3-aminopropanesulfonic acid sodium-potassium salt, [(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]-1-propanesulfonic acid sodium-potassium salt
Empirical Formula (Hill Notation):
C7H16NNaO6S
CAS Number:
91000-53-2
Molecular Weight:
265.26
MDL number:
MFCD00069903
UNSPSC Code:
12161700
PubChem Substance ID:
24899903
NACRES:
NA.25

Quality Level

200

Assay

≥99% (titration)

form

crystalline powder

impurities

≤1% water content (Karl Fischer)

useful pH range

7.7-9.1

pKa (25 °C)

8.4

solubility

water: 0.33 g/mL, clear to slightly hazy, colorless

cation traces

Pb: ≤5 ppm

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].OCC(CO)(CO)NCCCS([O-])(=O)=O

InChI

1S/C7H17NO6S.Na/c9-4-7(5-10,6-11)8-2-1-3-15(12,13)14;/h8-11H,1-6H2,(H,12,13,14);/q;+1/p-1

InChI key

FEGYIWVHCSRXCG-UHFFFAOYSA-M

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General description

N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS) is a zwitterionic ionic buffer that is widely used in capillary electrophoresis. As one of the key biological buffers, it maintains the required pH of the medium and exhibits various properties such as high water soluble, highly inert, and stable nature and extremely low absorbance of ultraviolet-visible (UV-vis) light. TAPS buffer also aids in stabilizing lysozyme against thermal denaturation. It is widely used in biology and biochemistry.

Application

TAPS sodium salt has been used along with filtered natural sea water (FSW) to stain live or fixed embryos.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The effects of biological buffers TRIS, TAPS, TES on the stability of lysozyme
Pannuru P, et al.
International Journal of Biological Macromolecules, 112, 720-727 (2018)
Capillary electrophoretic study of interactions of metal ions with crown ethers, a sulfated beta-cyclodextrin, and zwitterionic buffers present as additives in the background electrolyte
Muzikar M, et al.
Electrophoresis, 23(12) , 1796-802 (2002)
Buffer Standards for the Physiological pH of the Zwitterionic Compound, TAPS, From 5 to 55? C
Roy L N, et al.
Journal of Solution Chemistry, 35, 551-566 (2006)

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