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T0438

Sigma-Aldrich

Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse

clone 9016, purified immunoglobulin, lyophilized powder

Synonym(s):

Anti-TGF-β1, CEDLAP, DPD1, latency-associated peptide

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About This Item

MDL number:
MFCD00802737
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9016, monoclonal

form

lyophilized powder

species reactivity

mouse, human, rat

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

P01137

application(s)

research pathology

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TGFB1(7040)
mouse ... Tgfb1(21803)
rat ... Tgfb1(59086)

General description

Transforming Growth Factor-β1 (TGFβ1) is mapped to human chromosome 19q13.2.
Transforming growth factor-β (TGF-β) belongs to the TGF-β superfamily of secreted proteins. TGF-β 1 exists as three isoforms TGF-β 1, TGF-β 2 and TGF-β 3. The TGF-β family is involved in a variety of signaling pathways that involve serine/threonine protein kinases. TGF-β acts through binding to three transmembrane receptors in mammals, type I, type II and type III receptors. The important pathways regulated by TGF-β are the SMAD, BMP, MAPK and PI3K/Akt pathways. TGF-β 1 is involved in cell and tissue differentiation, proliferation, inflammation, migration of monocytes, neutrophils and chemotaxis. Deregulation of TGF-β signaling has been implicated in many inflammation and autoimmune diseases. Monoclonal Anti-Transforming Growth Factor-β 1 antibody specifically reacts with human, mouse and rat TGF-β 1. The antibody shows less than 2% cross reactivity with TGF-β3 and TGF-β5 in ELISA.

Specificity

By capture ELISA, <2% cross-reactivity is observed with TGF-β3 and TGF-β5.

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant TGF- beta 1 and latent TGF- beta 1

Application

Recommended concentrations:
Western Blot 1 ug/mL (under non-reducing conditions only)
Immunohistochemistry 3-25 μg/mL
Flow Cytometry 2.5 μg/106 cells
ELISA capture 2-8 μg/mL
ELISA detection 0.1-0.4 μg/mL
Neutrilization 0.3-1.0 μg/mL
Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse has been used in:
  • immunohistochemical detection of TGFβ1 in smooth muscle(2) and liver sections
  • western blotting and ELISA of brain specimens
  • immunoblot detection of TGFβ1 in macrophages

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Other Notes

Protein A or G purified from hybridoma culture supernatant.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLBX6210
SLBW5525
SLBS0285V
SLBQ2671V
SLBN9788V

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Can Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, be used to neutralize the biological activity of TGF-ß1?

    Yes, this antibody will neutralize TGF-ß1.  

  7. Can Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, be used for immunohistochemistry with frozen and/or formalin-fixed paraffin-embedded sections?

    This antibody can be used with both formalin-fixed paraffin-embedded (FFPE) tissue sections and frozen sections.

  8. Will Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, work for intracellular flow cytometry?

    This antibody can be used for intracellular flow cytometry. The cells should be fixed with 4% paraformaldehyde and permeabilized with a 0.1% saponin-containing buffer.

  9. How is Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, different than T0688,  Anti-Transforming Growth Factor-?1 antibody produced in chicken?

    These two antibodies are very similar in regards to the applications they are suitable for. They do differ in a few key areas. T0688 only recognizes active TGF-ß1 while T0438 recognizes both active and latent TGF-ß1. Also, T0438 is a mouse monoclonal antibody and T0688 is a chicken polyclonal antibody.

  10. What is the molecular weight of Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse,?

    TGF-ß1 is a disulfide-linked homodimer of two 12.5 kDa subunits. Under non-reducing conditions, the T0438 antibody should detect a protein at 25 kDa.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Alba Di Pardo et al.
Molecular brain, 6, 55-55 (2013-12-18)
Huntington Disease (HD) is a neurodegenerative disorder resulting from the expansion of polyglutamine stretch in the huntingtin protein (Htt). Mutant HTT (mHtt) leads to progressive impairment of several molecular pathways that have been linked to disease pathogenesis. Defects in the
Gangwen Han et al.
International journal of biological sciences, 8(2), 228-235 (2012-01-19)
TGFβ1 was initially identified as a potent chemotactic cytokine to initiate inflammation, but the autoimmune phenotype seen in TGFβ1 knockout mice reversed the dogma of TGFβ1 being a pro-inflammatory cytokine to predominantly an immune suppressor. The discovery of the role
The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4.
Fayed MR, et al.
Drug Discoveries & Therapeutics, 12(2), 58-67 (2018)
Huafeng Wei et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 35(3), 267-275 (2012-03-17)
Cells from many tumors produce transforming growth factor (TGF)-β which facilitates their escape from control by the immune system. We previously reported that nonimmunogenic cells from either of 2 transplantable mouse tumors became effective as therapeutic tumor vaccines after lentivirus-mediated
Margaret M Njoroge et al.
The American journal of tropical medicine and hygiene (2020-12-31)
Intranasal instillation of SE36, a malaria vaccine candidate antigen, in lactating BALB/c female mice resulted in the appearance of the antigen in breast milk as demonstrated by sandwich ELISA and Western blot. Pups born of immunologically naive mice and breastfed
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