T0157
Tumor Necrosis Factor-α human
TNF-α, recombinant, expressed in yeast, buffered aqueous solution, suitable for cell culture
Synonym(s):
hTNF-α, TNF-α
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
biological source
human
recombinant
expressed in yeast
Assay
≥95% (SDS-PAGE)
form
buffered aqueous solution
potency
>1x108 units/mg ED50
quality
endotoxin tested
specific activity
≥2 × 107 U/mg
mol wt
~17.4 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
0.1-10 mg/mL
technique(s)
cell culture | mammalian: suitable
impurities
<10 EU/mLtested (LAL test)
color
colorless
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... TNF(7124)
Looking for similar products? Visit Product Comparison Guide
General description
TNFα (tumor necrosis factor α) belongs to the TNF superfamily and is mapped to human chromosome 6p21.33.
Application
Tumor Necrosis Factor-α human has been used to study the differential regulation of microRNA-146a , CFH (complement factor-H), IRAK-1 (interleukin-1 receptor associated kinase-1) and tetraspanin-12 in primary brain cell cultures in presence of TNF-α. To determine the intercellular adhesion molecule-1 (ICAM1) expression using ELISA.
Biochem/physiol Actions
TNF-α is associated with formation, growth and destabilization of atherosclerotic plaques. TNF-α is involved in the disease progression of osteoarthritis.
Tumore necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is N-glycosylated.
Physical form
Solution in phosphate buffered saline containing 1 mg/ml bovine serum albumin.
Analysis Note
The cytolytic activity of TNF-α against WEHI 164 cells has been measured in culture using an MTT toxicity assay. The ED50 is defined as the concentration of TNF-alpha that mediates half-maximal cytotoxicity in the presence of 1 micro-g/ml actinomycin D.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Casper Steenholdt et al.
The American journal of gastroenterology, 109(7), 1055-1064 (2014-05-07)
Cost-effective guidance of therapeutic strategy in Crohn's disease patients with secondary infliximab (IFX) treatment failure may be achieved by serum IFX and anti-IFX antibody (Ab) measurements by radioimmunoassay (RIA). This study investigated implications of using other techniques for this purpose.
Fen Li et al.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 40(9), 945-954 (2015-09-27)
To investigate the association of TNF-α-308 gene polymorphism with the risk of RA in Hunan's Han Chinese population. A total of 112 patients with RA from Han Chinese population in Hunan were recruited, along with 129 healthy controls. TNF-α-308 (rs1800629)
Fabrícia Lima Fontes et al.
BMC medical genetics, 16, 70-70 (2015-09-01)
Bacterial meningitis (BM) is an infectious disease that results in high mortality and morbidity. Despite efficacious antibiotic therapy, neurological sequelae are often observed in patients after disease. Currently, the main challenge in BM treatment is to develop adjuvant therapies that
David J Konieczkowski et al.
Cancer discovery, 4(7), 816-827 (2014-04-29)
Most melanomas harbor oncogenic BRAF(V600) mutations, which constitutively activate the MAPK pathway. Although MAPK pathway inhibitors show clinical benefit in BRAF(V600)-mutant melanoma, it remains incompletely understood why 10% to 20% of patients fail to respond. Here, we show that RAF
Juan L García-Rodríguez et al.
Hepatology (Baltimore, Md.), 59(5), 1972-1983 (2013-12-18)
Sirtuin1 (SIRT1) regulates central metabolic functions such as lipogenesis, protein synthesis, gluconeogenesis, and bile acid (BA) homeostasis through deacetylation. Here we describe that SIRT1 tightly controls the regenerative response of the liver. We performed partial hepatectomy (PH) to transgenic mice
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service