SRP8023
PARG human
recombinant, expressed in Sf21 cells, His tagged, >95% (SDS-PAGE)
Synonym(s):
FNDC5 (cleaved), Poly(ADP-ribose) glycohydrolase
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About This Item
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biological source
human
recombinant
expressed in Sf21 cells
tag
His tagged
Assay
>95% (SDS-PAGE)
form
liquid
mol wt
~110 kDa by SDS-PAGE
packaging
pkg of 2 μg
concentration
≥0.2 mg/mL
technique(s)
cell based assay: suitable
color
clear
solubility
water: soluble
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PARG(8505)
General description
Research area: Cell signaling
Application
PARG human has been used in the digestion of sciatic nerve sections, in co-immunoprecipitation, in in vitro glycohydrolase activity assay
Biochem/physiol Actions
Poly(ADP-ribose) glycohydrolase(PARG) hydrolyzes poly(ADP-ribose) at glycosidic(1′′-2′′) linkage of ribose-ribose bond to produce free ADP-ribose. It is an endo- and exo-glycosidase. PARG plays arole in DNA repair and replication. Inhibited or depleted PARG in cells aremore prone to DNA-damaging agents. PARG also averts the accumulation of PAR inthe cytoplasm and parthanatos, a caspase-independent PAR-mediated type of celldeath.
Physical form
Solution in 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2% NP-40, 50 mM imidazole and 10% glycerol.
Other Notes
Human PARG is fused to a His-tag.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Sara C Larsen et al.
Cell reports, 24(9), 2493-2505 (2018-08-30)
ADP-ribosylation (ADPr) is a reversible posttranslational modification involved in a range of cellular processes. Here, we report system-wide identification of serine ADPr in human cells upon oxidative stress. High-resolution mass spectrometry and unrestricted data processing confirm that serine residues are
Daniel Harrision et al.
Frontiers in molecular biosciences, 7, 191-191 (2020-10-03)
Poly(ADP-ribose) polymerases (PARPs) are a family of enzymes that catalyze the addition of poly(ADP-ribose) (PAR) subunits onto themselves and other acceptor proteins. PARPs are known to function in a large range of cellular processes including DNA repair, DNA replication, transcription
Sara C Buch-Larsen et al.
Cell reports, 32(12), 108176-108176 (2020-09-24)
ADP-ribosylation (ADPr) is a post-translational modification that plays pivotal roles in a wide range of cellular processes. Mass spectrometry (MS)-based analysis of ADPr under physiological conditions, without relying on genetic or chemical perturbation, has been hindered by technical limitations. Here
Xiao-Nan Zhang et al.
Nature communications, 10(1), 4196-4196 (2019-09-15)
Nicotinamide adenine dinucleotide (NAD+)-dependent ADP-ribosylation plays important roles in physiology and pathophysiology. It has been challenging to study this key type of enzymatic post-translational modification in particular for protein poly-ADP-ribosylation (PARylation). Here we explore chemical and chemoenzymatic synthesis of NAD+
Yongjia Duan et al.
Cell research, 29(3), 233-247 (2019-02-08)
Mutations in RNA-binding proteins (RBPs) localized in ribonucleoprotein (RNP) granules, such as hnRNP A1 and TDP-43, promote aberrant protein aggregation, which is a pathological hallmark of various neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Protein
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