SRP8012
Jagged-1 (human): FC (human)
recombinant, expressed in HEK 293 cells, >90% (SDS-PAGE)
Synonym(s):
JAG1, Protein jagged-1, Tweak-receptor, hJ1
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in HEK 293 cells
Assay
>90% (SDS-PAGE)
form
liquid
mol wt
~150 kDa by SDS-PAGE
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
≥0.2 mg/mL
technique(s)
ligand binding assay: suitable
impurities
<0.1 EU/μg endotoxin, tested
suitability
suitable for molecular biology
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... JAG1(182)
General description
Jagged-1 is a ligand for multiple Notch receptors and involved in the mediation of Notch signaling. It is involved in cell-fate decisions during hematopoiesis. Is involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Defects in Jagged-1 are the cause of Alagille syndrome type 1 (ALGS1) and tetralogy of Fallot (TOF).
Jagged-1, encoded by the gene JAG1, is a Serrate homolog found in vertebrates. It is expressed by both primitive and mature human hematopoietic cells. The encoded protein contains EGF (epidermal growth factor) repeats, transmembrane domain and a cysteine-rich region in between the two.
Application
Jagged-1 has been used to activate Notch1 signaling pathway that modulates cardiac AMPK (5′ AMP-activated protein kinase) signaling cascade. Jagged-1 (human): FC (human) has been used to determine the cardiac substrate (fatty acid and glucose) metabolism in working heart model.
Biochem/physiol Actions
The encoded protein is a transmembrane-type ligand for Notch family receptor and is capable of inducing Notch signaling, which participates in embryogenesis, tissue regeneration, and carcinogenesis. Jagged-1 is a WNT-dependent Notch signaling activator that is involved in the maintenance of homeostasis of stem and progenitor cells. Jagged proteins are found to be involved in development and carcinogenesis. Overexpression of Jagged-1 has been observed in cervical and breast cancers.
Physical form
Solution in PBS.
Other Notes
Signal peptide and extracellular domain of human Jagged-1 (HJ1; CD339) (aa 1-1067) are fused at the C-terminus to the Fc portion of human IgG1.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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B Varnum-Finney et al.
Blood, 91(11), 4084-4091 (1998-05-30)
We examined the expression of two members of the Notch family, Notch-1 and Notch-2, and one Notch ligand, Jagged-1, in hematopoietic cells. Both Notch-1 and Notch-2 were detected in murine marrow precursors (Lin-Sca-1+c-kit+). The Notch ligand, Jagged-1, was not detected
Notch ligand, JAG1, is evolutionarily conserved target of canonical WNT signaling pathway in progenitor cells.
Katoh M and Katoh M
International Journal of Molecular Medicine, 17, 681-685 (2006)
Hui Yang et al.
Biochemical pharmacology, 108, 47-57 (2016-03-27)
AMP-activated protein kinase (AMPK) signaling pathway plays a pivotal role in intracellular adaptation to energy stress during myocardial ischemia. Notch1 signaling in the adult myocardium is also activated in response to ischemic stress. However, the relationship between Notch1 and AMPK
High-level coexpression of JAG1 and NOTCH1 is observed in human breast cancer and is associated with poor overall survival.
Reedijk M
Cancer Research, 65, 8530-8537 (2005)
The notch ligand jagged-1 represents a novel growth factor of human hematopoietic stem cells.
Karanu FN
The Journal of Experimental Medicine, 192, 1365-1372 (2000)
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