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SRP8003

Sigma-Aldrich

Clusterin (nuclear form) mouse

recombinant, expressed in E. coli, His tagged, >90% (SDS-PAGE)

Synonym(s):

Apolipoprotein J, CLI, Lymphocyte antigen 52 (Ly-52), SGP-2, TRPM-2

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

tag

His tagged

Assay

>90% (SDS-PAGE)

form

liquid

mol wt

~45 kDa by SDS-PAGE

packaging

pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

≥0.2 mg/mL

impurities

<1 EU/μg endotoxin, tested

color

clear

UniProt accession no.

Q06890

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Clu(12759)

General description

Clusterin is expressed in all tissues. However, it is strongly expressed in the central nervous system and plasma. Clusterin mainly protects against heat and other stress. It is also referred as chaperone protein and stress-inducible biomarker. Clusterin exists in two isoforms, one is a secretory form and other is a nuclear form. The secreted form of the protein is a 80kDa disulfide-linked heterodimer of α and β subunits (produced by internal cleavage). The alternatively spliced inactive cytosolic form is changed to a truncated mature nuclear isoform via ionizing irradiation.

Biochem/physiol Actions

Clusterin is involved in tissue remodeling, cell death, lipid transport, complement-associated cell lysis, cancer and neurological damage. It is strongly expressed in Alzheimer′s and treatment-resistant cancer. High levels of clusterin are observed in ischemic heart disease and diabetic conditions. It also protects kidney from fibrosis. Nuclear form of clusterin works as a pro-death signal, and suppresses cell growth and survival. The secreted form of clusterin has extracellular chaperone and anti-apoptotic activities.

Physical form

Solution in 55 mM TRIS-HCl, pH 8.2, containing 150 mM NaCl.

Other Notes

Mouse nuclear clusterin (aa 22-448) is fused at the C-terminus to a His-tag.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Ca2+ depletion induces nuclear clusterin, a novel effector of apoptosis in immortalized human prostate cells.
Caccamo AE, et al.
Cell Death and Differentiation, 12, 101-101 (2005)
Clusterin/ApoJ enhances central leptin signaling through Lrp2-mediated endocytosis.
Byun K, et al.
The Embo Journal, 15, 801-801 (2014)
Clusterin is a potential lymphotoxin beta receptor target that is upregulated and accumulates in germinal centers of mouse spleen during immune response.
Afanasyeva MA, et al.
PLoS ONE, 9, e98349-e98349 (2014)
Clusterin Modulates Allergic Airway Inflammation by Attenuating CCL20-Mediated Dendritic Cell Recruitment.
Hong GH, et al.
Journal of Immunology, 196, 2021-2021 (2001)
Synthesis and functional analyses of nuclear clusterin, a cell death protein.
Leskov KS, et al.
The Journal of Biological Chemistry, 278, 11590-11590 (2003)
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