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SRP6455

Sigma-Aldrich

EpCAM/CD326 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

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Synonym(s):
DIAR5, EGP2, EGP314, EGP40, ESA, GA733-2, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK1, TACSTD1, TROP1
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 29 kDa (after removal of signal peptide.)
observed mol wt 33-36 kDa by SDS-PAGE (DTT-reduced. rh EpCAMProtein migrates as poly peptide due to glycosylation. Gln24 is the predicted N-terminal.)

packaging

pkg of 10 and 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P16422

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EPCAM(4072)

General description

Epithelial cell adhesion molecule (EpCAM) is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A (mAb) antigen. The gene encoding EpCAM is localized on human chromosome 2p21.

Biochem/physiol Actions

Epithelial cell adhesion molecule (EpCAM) is intricately linked with the cadherin-catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The protein has several cellular functions, like epithelial-specific cell-adhesion, cell signaling, migration, proliferation and differentiation. It is expressed in most epithelial malignancies (like epithelial ovarian cancer) and was reported as a tumor marker or a candidate of molecular targeting therapy. EpCAM cross signaling with N-cadherin involves phosphoinositide-3-kinase (Pi3K), resulting in the abrogation of the cadherin adhesion complexes in epithelial cells.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

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The Epithelial Cell Adhesion Molecule (Ep-CAM) as a Morphoregulatory Molecule Is a Tool in Surgical Pathology
Manon J. Winter
The American Journal of Pathology, 163(6), 2139-2148 (2003)
The evolving roles of canonical WNT signaling in stem cells and tumorigenesis: implications in targeted cancer therapies
Ke Yang
Laboratory Investigation; a Journal of Technical Methods and Pathology, 96, 116-136 (2016)
Epithelial cell adhesion molecule expression (CD326) in cancer: a short review.
Patriarca C
Cancer Treatment Reviews, 38(1), 68-75 (2012)
Mamata Sivagnanam et al.
Gastroenterology, 135(2), 429-437 (2008-06-24)
Congenital tufting enteropathy (CTE) is a rare autosomal recessive diarrheal disorder presenting in the neonatal period. CTE is characterized by intestinal epithelial cell dysplasia leading to severe malabsorption and significant morbidity and mortality. The pathogenesis and genetics of this disorder
Overexpression of epithelial cell adhesion molecule (Ep-CAM) is an independent prognostic marker for reduced survival of patients with epithelial ovarian cancer.
Spizzo G
Gynecologic Oncology, 103(2), 483-488 (2006)
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