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Safety Information

SRP6451

Sigma-Aldrich

Fc gamma RIIIB/CD16b human

recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE)

Synonym(s):

CD16B, FCG3, FCG3B, FCGR3, FCGR3B, IGFR3

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥98% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 21.7 kDa
observed mol wt 38-50 kDa (DTT-reduced. Protein migrates due to glycosylation. Gly 17 is the predicted N-terminal.)

packaging

pkg of 10 and 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

O75015

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FCGR3B(2215)

General description

Cluster of differentiation 16 (CD16) is a low affinity type 1 transmembrane Fc receptor. It possesses two Ig-like extracellular domains. Cd16 has been identified as two isoforms, Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner. CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16B also known as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils.

Biochem/physiol Actions

Cluster of differentiation 16B (CD16B) or Fcγ RIIIB (FcγRIIIb) is the low affinity receptor for the Fc region of immunoglobulin γ. It binds complexed or aggregated IgG and also to monomeric IgG. Contrary to CD16A, CD16B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation, which does not activate neutrophils.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
9I127446

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The Human Low Affinity Immunoglobulin G Fc Receptor III-A and III-B Genes MOLECULAR CHARACTERIZATION OF THE PROMOTER REGIONS.
Gessner J E, et al.
The Journal of Biological Chemistry, 270(3), 1350-1361 (1995)
Michael T L and Angus W T
Natural Killer Cells (2010)

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