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SRP6448

Sigma-Aldrich

Fas Ligand/FasL human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

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Synonym(s):
ALPS1B, APT1LG1, CD178, CD95-L, CD95L, FASL, FASLG, Fas ligand, TNFSF6
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (N-terminus)

Assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 17.7 kDa
observed mol wt 25-32 kDa by SDS-PAGE (reducing) (His is the predicted N-terminal .)

packaging

pkg of 10 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P48023

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FASLG(356)

General description

Fas ligand also known as FasL, CD178, CD95L, or TNFSF6, is a 40 kDa homotrimeric type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. The FASL gene was mapped to human chromosome 1q24.3. It spans a length of 8 kb and contains 4 exons. It is found to be expressed on T and NK cells that are activated.

Biochem/physiol Actions

The binding of Fas ligand to its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Its functions include T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lympho proliferative syndrome (ALPS), a childhood disorder of apoptosis.

Physical form

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Metalloproteinase-mediated release of human Fas ligand.
Kayagaki N, et al.
The Journal of Experimental Medicine, 182(6), 1777-1783 (1995)
Insights into the molecular regulation of FasL (CD178) biology.
Lettau M, et al.
European Journal of Cell Biology, 90(6-7), 456-466 (2011)
Statistical colocalization of genetic risk variants for related autoimmune diseases in the context of common controls.
Fortune M D, et al.
Nature Genetics, 47(7), 839-839 (2015)
Dominant interfering Fas gene mutations impair apoptosis in a human autoimmune lymphoproliferative syndrome.
Fisher G H, et al.
Cell, 81(6), 935-946 (1995)
T Takahashi et al.
International immunology, 6(10), 1567-1574 (1994-10-01)
Fas ligand (FasL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. In this report, we isolated the chromosomal gene for human FasL. The human

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