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SRP6293

Sigma-Aldrich

IL-15 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

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Synonym(s):
Interleukin-15
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 12.8 kDa (no tag)
observed mol wt 16 kDa (DTT-reduced. Protein migrates due to glycosylation. Asn 49 is the predicted N-terminus .)

packaging

pkg of 10 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P40933

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL-15(3600)

General description

IL15 (interleukin 15) gene is localized to human chromosome 4q31, composed of eight exons, and the encoded protein is a short-chain α-helical bundle type 1 cytokine. IL15, along with its receptor IL15Rα, are co-expressed majorly by activated monocytes and DCs (dendritic cells).

Biochem/physiol Actions

IL15 (interleukin 15) and IL2 are the only two known cytokines till date that bind to T-cells through IL-2Rβ (IL2 receptor β). It interacts with lymphocytes via the heterotrimeric IL-15 receptor (IL-15R) composed of IL-15Rα, IL-2 receptor β (IL-2Rβ), and IL-2 receptor γ (IL-2Rγ). Since, IL-2R and IL-15R have common subunits, it is supposed that IL2 and IL15 perform similar functions, such as induction of lymphocyte activation and proliferation in vitro, and apoptosis of activated lymphocytes (AICD). However, unlike IL2, IL15 mimics the function of IFN I (interferon) by potently and selectively activating memory-phenotype CD44hi CD8+ cells.

Physical form

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 100 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

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The shared and contrasting roles of interleukin-2 (IL-2) and IL-15 in the life and death of normal and neoplastic lymphocytes: implications for cancer therapy
Waldmann T A
Cancer Immunology, Immunotherapy, 3(3), 219- 227 (2015)
Potent and selective stimulation of memory-phenotype CD8+ T cells in vivo by IL-15.
Zhang X, et al.
Immunity, 8(5), 591-599 (1998)
IL-15 receptor maintains lymphoid homeostasis by supporting lymphocyte homing and proliferation.
Lodolce JP, et al.
Immunity, 9(5), 669-676 (1998)

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