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SRP6287

Sigma-Aldrich

LIF human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

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Synonym(s):
CDF, DIA, HILDA, LIF, MLPLI
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 19.9 kDa (with Gly-Pro at the N- terminus)
observed mol wt 35-45 kDa (DTT-reduced.Ser 23 is the predicted N-terminus .)

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P15018

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... LIF(3976)

General description

Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line.

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts, and inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B-cell lymphoma-extra large) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.

Physical form

Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Protective effects of leukemia inhibitory factor against oxidative stress during high glucose-induced apoptosis in podocytes.
Xu J et al
Cell Stress & Chaperones, 17(4), 485-493 (2012)
Leukemia inhibitory factor (LIF) and its biological activity.
Chodorowska G et al
Annales Universitatis Mariae Curie-Sklodowska. Sectio D: Medicina, 59(2), 189-193 (2004)
Complex conserved organization of the mammalian leukemia inhibitory factor gene: regulated expression of intracellular and extracellular cytokines.
Haines BP et al
Journal of Immunology, 162(8), 4637-4646 (1999)
The gp130 receptor cytokine family: regulators of adipocyte development and function.
White UA and Stephens JM
Current Pharmaceutical Design, 17(4), 340-346 (2011)

Articles

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

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