SRP6263
PLGF/PIGF2/PGF human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym(s):
PGFL, SHGC-10760
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
calculated mol wt 20.2 kDa, observed mol wt 28-33 kDa (DTT-reduced. Protein migrates due to glycosylation. Arg 29 is the predicted N-terminus.)
Impurities:
<1 EU/μg endotoxin (LAL test)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
calculated mol wt 20.2 kDa, observed mol wt 28-33 kDa (DTT-reduced. Protein migrates due to glycosylation. Arg 29 is the predicted N-terminus.)
packaging
pkg of 10 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... PLGF(5228)
General description
Placental growth factor (PLGF) exists in two isoforms namely PLGF1 and PLGF2. This protein is a homodimeric glycoprotein with molecular weight of 46–50kDa and is a member of the VEGF (vascular endothelial growth factor) subfamily. PLGF is encoded by the gene mapped to human chromosome 14q24-q31 regions. PLGF is expressed mainly in the placental trophoblast during pregnancy and is also present in various other tissues such as the villous trophoblast.
Biochem/physiol Actions
PLGF might enhance the production of peripheral blood mononuclear cell-vascular endothelial growth factor (PBMC-VEGF). Consequently, PlGF might facilitate VEGF production in the inflammatory joint. Soluble fms related tyrosine kinase 1 (Flt-1), which is considered to be an anti-angiogenic factor interacts with and inhibit the activity of PLGF. Therefore, PLGF level is lowered and the Flt-1 level is increased in placental tissue of women with pre-eclampsia (PE). PLGF is associated with the pathogenesis of rheumatoid arthritis (RA) and various other arthropathies. PLGF acts as a ligand for VEGFR-1 and is implicated in various physiological functions such as tumor angiogenesis, wound healing and hematopoietic progenitors recruitment.
Physical form
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
监管及禁止进口产品
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Effect of antihypertensive therapy with alpha methyldopa on levels of angiogenic factors in pregnancies with hypertensive disorders.
Khalil A
PLoS ONE, 3(7), e2766-e2766 (2008)
PlGF blockade does not inhibit angiogenesis during primary tumor growth.
Bais C
Cell, 141(1), 166-177 (2010)
Role of placenta growth factor (PIGF) in human extravillous trophoblast proliferation, migration and invasiveness.
Athanassiades A and Lala PK
Placenta, 19(7), 465-473 (1998)
Placenta growth factor (PlGF) induces vascular endothelial growth factor (VEGF) secretion from mononuclear cells and is co-expressed with VEGF in synovial fluid.
Bottomley MJ
Clinical and Experimental Immunology, 119(1), 182-188 (2000)
Assignment of vascular endothelial growth factor (VEGF) and placenta growth factor (PLGF) genes to human chromosome 6p12-p21 and 14q24-q31 regions, respectively.
M G Mattei et al.
Genomics, 32(1), 168-169 (1996-02-15)
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