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Safety Information

SRP6217

Sigma-Aldrich

Fetuin A/ASHG human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):

Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

45-55 kDa

packaging

pkg of 10 and 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FETUA(197)

General description

Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuin A/AHSG is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β and BMP signaling proteins. Recombinant human Fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant Fetuin-A migrates at an apparent molecular weight of 45 – 55 kDa by SDS-PAGE run under non-reducing conditions.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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