SRP6169
IL-1β human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym(s):
Catabolin, Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Lymphocyte-activating factor (LAF), Mononuclear cell factor (MCF)
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About This Item
Form:
lyophilized
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Mol wt:
(18 and 25 kDa, monomer, glycosylated)
biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
(18 and 25 kDa, monomer, glycosylated)
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... IL1B(3553)
General description
Interleukin-1β (IL1B) is produced by activated macrophages. It is synthesized as pro-IL-1β which is changed to active form IL-1β with the help of pro-inflammatory protease caspase-1. IL-1β belongs to the IL-1 gene family. It is a proinflammatory cytokine. The gene is mapped to human chromosome 2q14. In comparison with the Escherichia coli expressed protein, IL-1β is 50% more potent in promoting human CD4+ T cell differentiation into Th17 (T helper) cells. This cytokine is produced in a serum free, chemically defined media.
Application
- IL-1β (IL1B) has been used to check the expression of NGAL (neutrophil gelatinase-associated lipocalin) in epithelial cells after stimulation with proinflammatory cytokines.
- It has been used to check anti-inflammatory activities of N-Acetylglucosamine in human chondrocytes.
- It has been used to create inflammatory endothelium in human coronary artery endothelial cells.
Biochem/physiol Actions
IL-1β (IL1B) stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation and fibroblast growth factor activity. IL-1β proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1β is present in chronic inflamed tissues and also in tissues going for fibrogenesis. It brings neutrophil and macrophage to the site of infection. Mutations in IL-1β gene are linked with renal manifestations and renal sequelae in Henoch-Schönlein purpura. It is also associated with destruction of β-cell in type 1 diabetes.
Physical form
Lyophilized from a PBS solution.
Preparation Note
Centrifuge the vial prior to opening.
Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Proinflammatory cytokines detectable in synovial fluids from patients with temporomandibular disorders.
Takahashi T
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 85, 135-141 (1998)
Virus infection activates IL-1 beta and IL-18 production in human macrophages by a caspase-1-dependent pathway.
Pirhonen J
Journal of Immunology, 162, 7322-7329 (1999)
Differential regulation of monocyte matrix metalloproteinase and TIMP-1 production by TNF-alpha, granulocyte-macrophage CSF, and IL-1 beta through prostaglandin-dependent and -independent mechanisms.
Zhang Y
Journal of Immunology, 161, 3071-3076 (1998)
Neutrophil gelatinase-associated lipocalin is up-regulated in human epithelial cells by IL-1 beta, but not by TNF-alpha.
Cowland JB
Journal of Immunology, 171, 6630-6639 (2003)
Microbubbles targeted to intercellular adhesion molecule-1 bind to activated coronary artery endothelial cells.
Villanueva FS
Circulation, 98, 1-5 (1998)
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