SRP6109
α-Enolase human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym(s):
2-phospho-D-glycerate hydro-lyase, Alpha-enolase, ENO1, Enolase 1, Non-neural enolase
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biological source
human
recombinant
expressed in E. coli
Assay
≥90% (SDS-PAGE)
form
liquid
mol wt
47.1 kDa (434 aa, 1-434 aa)
packaging
pkg of 100 μg
technique(s)
activity assay: suitable
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... ENO1(2023)
General description
α-Enolase is encoded by the gene mapped to human chromosome 1p36.3-p36.2.
α-Enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. It is predominantly expressed in the early stages of embryonic development. The enzyme consists of two subunits of 433 amino-acids each. Each subunit has a molar mass of 47kDa. The dimer is stabilized in the presence of magnesium.
Application
α-Enolase human has been used in in vitro kinase activity assay.
Biochem/physiol Actions
α-Enolase catalyzes the dehydration of 2-phosphoglycerate to phosphoenolpyruvate. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of α-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an auto antigen associated with Hashimoto′s encephalopathy and severe asthma.
Physical form
1 mg/mL solution in 20 mM Tris-HCl (pH 7.5) containing 10% glycerol and 1 mM MgSO4.
Preparation Note
Centrifuge the vial prior to opening.
Other Notes
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Hye-Ah Lee et al.
Yonsei medical journal, 49(6), 923-930 (2008-12-25)
A possible involvement of autoimmune mechanism in the pathogenesis of bronchial asthma has been proposed. Recently, alpha-enolase protein was identified as a major autoantigen recognized by circulating IgG autoantibodies in patients with severe asthma. To evaluate a possible pathogenetic significance
a-Enolase: a promising therapeutic and diagnostic tumor target.
Capello M
FEBS Journal, 278, 1064-1074 (2011)
Phosphoproteomic analysis of interacting tumor and endothelial cells identifies regulatory mechanisms of transendothelial migration.
Locard-Paulet M, et al.
Science Signaling, 9(414), ra15-ra15 (2016)
Effects of pulsed electromagnetic fields on human osteoblastlike cells (MG-63): a pilot study.
Sollazzo V, et al.
Clinical Orthopaedics and Related Research, 468(8), 2260-2277 (2010)
Alpha-enolase: a target of antibodies in infectious and autoimmune diseases.
Terrier B
Autoimmunity Reviews, 6, 176-182 (2007)
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