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Safety Information

SRP6055

Sigma-Aldrich

Granzyme A human

recombinant, expressed in mammalian cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(s):

CTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in mammalian cells

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

26.9 kDa

packaging

pkg of 10 μg

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GZMA(3001)

General description

Granzyme A is a member of the granzyme family. Granzyme A is the most abundant serine protease in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A has a specific function in CTL and NK cells. It induces caspase-independent cell death when introduced into target cells by perforin. Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1-26), a propeptide (residues 27-28) and a mature chain (residues 29-262).

Physical form

Lyophilized from a 0.2 μm filtered solution of 20 mM MES and150 mM NaCl, pH 5.5.

Preparation Note

Centrifuge the vial prior to opening.

Reconstitution

Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers.

Other Notes

Recombinant Human Granzyme A is produced with mammalian cells expression system. Target protein is expressed with sequence (Glu27-Val262) of Human granzyme a (Uniprot #P12544) fused with a polyhistidine tag at the C-terminus.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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