SRP5205
MBP, GST tagged human
recombinant, expressed in E. coli, ≥65% (SDS-PAGE), buffered aqueous glycerol solution
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About This Item
biological source
human
recombinant
expressed in E. coli
Assay
≥65% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
~36 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MBP(4155)
General description
Myelin basic protein (MBP), an important constituent of the myelin sheath of CNS (central nervous system), is an autoimmunogen. It is mapped to human chromosome 18q.
Biochem/physiol Actions
MBP or Myelin basic protein forms dimers across the cytoplasmic apposition during the formation of myelin. The region of the protein involved in folding, polymerization and substrate specificities is conserved in various species and it may have a specialized role in protein-lipid interactions in the myelin membrane. MBP is an efficient substrate for numerous protein kinases and share similarities with the MARCKS protein in terms of having extended conformations regulated by their environment, N-terminal modifications, a dual nature of interactions with lipids, and binding to actin and Ca2+-calmodulin. Myelin basic protein (MBP) is expected to play a vital role in demyelinating diseases such as MS (multiple sclerosis).
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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B E Chapman et al.
Advances in experimental medicine and biology, 100, 207-220 (1978-01-01)
High resolution 13C and 1H NMR spectra of myelin basic protein over a range of pH and concentrations indicate that intramolecular folding of the polypeptide chain occurs in aqueous solution in the region of residues 85 to 116. At pH
Molecular characterization of a patient with central nervous system dysmyelination and cryptic unbalanced translocation between chromosomes 4q and 18q
Gunn S R, et al.
American Journal of Medical Genetics. Part A, 120(1), 127-135 (2003)
Structure and developmental regulation of Golli-mbp, a 105-kilobase gene that encompasses the myelin basic protein gene and is expressed in cells in the oligodendrocyte lineage in the brain.
Campagnoni AT, et al.
Test, 268(7), 4930-4938 (1993)
G Harauz et al.
Molecular and cellular biochemistry, 209(1-2), 155-163 (2000-08-15)
Myelin basic protein (MBP) and myristoylated alanine-rich C-kinase substrate (MARCKS) are similar in terms of having extended conformations regulated by their environment (i.e., solubilised or lipid-associated), N-terminal modifications, a dual nature of interactions with lipids, binding to actin and Ca2+-calmodulin
Variation within MBP gene predicts disease course in multiple sclerosis
Zhou Y, et al.
Brain and Behavior, 7(4), e00670-e00670 (2017)
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