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Safety Information

SRP5104

Sigma-Aldrich

NFATC1, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

NF-ATC, NFAT2, NFATc

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About This Item

CAS Number:
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

~125 kDa

NCBI accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... NFATC1(4772)

General description

NFATC1 is a member of the NFAT family of proteins which are Ca2+/calcineurin-responsive transcription factors primarily recognized for their central roles in T lymphocyte activation and cardiac valve development. NFAT consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T-cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to the NFAT family of transcription factors play a central role in inducible gene transcription during immune response. The product of NFATC1 is an inducible nuclear component and functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Information

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Malte Buchholz et al.
The EMBO journal, 25(15), 3714-3724 (2006-07-29)
The nuclear factor of activated T cell (NFAT) proteins are a family of Ca2+/calcineurin-responsive transcription factors primarily recognized for their central roles in T lymphocyte activation and cardiac valve development. We demonstrate that NFATc1 is commonly overexpressed in pancreatic carcinomas
Bong Sook Jhun et al.
Biochemical and biophysical research communications, 351(4), 986-992 (2006-11-14)
AMP-activated protein kinase (AMPK) is a key regulator of energy homeostasis and its activation during T cell receptor stimulation has recently been reported. In this study, we examined the role of AMPK in interleukin (IL)-2 production in T cells. Inhibition

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