SRP4696
ApoE3 human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE), ≥90% (HPLC)
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Synonym(s):
Apolipoprotein E3
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biological source
human
recombinant
expressed in E. coli
Assay
≥90% (HPLC)
≥90% (SDS-PAGE)
form
lyophilized
mol wt
~34 kDa
packaging
pkg of 500 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
activity assay: suitable
impurities
endotoxin, tested
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... APOE(348)
General description
Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It′s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40-90% of the population. Recombinant human ApoE3 is a 34.0 kDa protein containing 299 amino acid residues. The recombinant ApoE3 was manufactured using animal origin free technology.
Application
Human ApoE has been used to study the role of apoE in the activity of LNP (lipid nanoparticles) in order to determine if ApoE has a role to play in the delivery of siRNA to hepatocytes.
Biochem/physiol Actions
ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Apart from participating in the metabolism of plasma lipoproteins, apo-E also interacts with several proteoglycans, such as heparin. The interaction of lipoproteins with proteoglycans in the walls of arteries has been associated with cholesterol deposition linked to atherosclerosis. It is found to be a constituent of several lipoproteins and serves as a ligand for the LDL (low density lipoprotein) receptor, thereby regulating cholesterol and triglyceride homeostasis.
Physical form
Sterile filtered and lyophilized from 20 mM sodium phosphate.
Reconstitution
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in 5 mM Sodium Phosphate pH 7.8 + 0.5 mM DTT to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
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Isotopic N,N-dimethyl leucine tags for absolute quantification of clusterin and apolipoprotein E in Alzheimer's disease.
Liu, et al.
Journal of proteomics, 257, 104507-104507 (2023)
Yuta Suzuki et al.
International journal of pharmaceutics, 510(1), 350-358 (2016-07-05)
Lipid nanoparticles (LNPs) represent the most advanced platform for the systemic delivery of siRNA. We have previously reported the discovery of novel ionizable lipids with asymmetric lipid tails, enabling potent gene-silencing activity in hepatocytes in vivo; however, the structure and
Emmanuel U Okoro et al.
Biochemical and biophysical research communications, 477(1), 123-128 (2016-06-15)
We previously reported that apolipoprotein E (apoE) upregulates ATP-binding cassette transporter A1 (ABCA1) transcription through phosphatidylinositol 3-kinase (PI3K). Here we demonstrate that treatment of murine macrophages with human apoE3 enhanced Akt phosphorylation, and upregulated ABCA1 protein and mRNA expression. Inhibition
Human apolipoprotein E. Determination of the heparin binding sites of apolipoprotein E3.
Weisgraber KH
The Journal of Biological Chemistry, 261, 2068-2076 (1986)
Fatemeh Nouri Emamzadeh et al.
Neuroscience letters, 618, 146-151 (2016-02-28)
Parkinson's disease is a progressive brain disorder due to the degeneration of dopaminergic neurons in the substantia nigra. The accumulation of aggregated forms of α-synuclein protein into Lewy bodies is one of the characteristic features of this disease although the
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