SRP3258
DKK-1 human
recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture
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Dickkopf-1, Dickkopf-related protein-1, SK
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biological source
human
recombinant
expressed in HEK 293 cells
Assay
≥97% (HPLC)
≥97% (SDS-PAGE)
form
lyophilized
mol wt
35-40 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... DKK1(22943)
General description
DKK-1 (dickkopf WNT signaling pathway inhibitor 1) is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. Recombinant human DKK-1 expressed in human 293 cells is a 35-40kDa glycoprotein containing 235 amino-acid residues.
Application
DKK-1 human has been used for the preincubation of human adipose-derived stromal/stem cells before growing them in neuron differentiation cocktail medium. It has also been used for the differentiation of induced pluripotent stem cells into photoreceptor progenitor cells.
Biochem/physiol Actions
DKK-1 (dickkopf WNT signaling pathway inhibitor 1) was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 play an important regulatory role in the Wnt/β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/β-catenin signaling system. It has been suggested that by inhibiting Wnt/β-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. In bladder cancer, DKK-1 is overexpressed in serum and might be associated with tumor progression. It is also associated with multiple myeloma and affects bone formation. Changes in serum levels of DKK1 is linked with inflammation in rheumatoid arthritis. In the process of development, tumorigenesis, and inflammation, DKK1 enhances angiogenesis.
Sequence
TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH
Physical form
Lyophilized from 1x PBS, pH 7.4.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Dickkopf1 is required for embryonic head induction and limb morphogenesis in the mouse.
Developmental Cell, 1, 423-434 (2001)
Differentiation of human adipose-derived stem cells into neuron-like cells which are compatible with photocurable three-dimensional scaffolds.
Tissue Engineering: Part A, 20, 1271-1284 (2014)
Downregulation of MicroRNA-152 contributes to high expression of DKK1 in multiple myeloma.
RNA Biology, 12, 1314-1322 (2015)
Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction.
Nature, 391, 357-362 (1998)
DKK1 expression by synovial fibroblasts in very early rheumatoid arthritis associates with lymphocyte adhesion in an in vitro flow co-culture system.
Arthritis Research & Therapy, 18, 14-14 (2016)
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