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SRP3256

TPO from rat

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

Key Documents

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Product Name

TPO from rat, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

biological source

rat

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.2 ng/mL ED50

mol wt

18.7 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

P49745

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... THPO(81811)

Related Categories

Biochem/physiol Actions

TPO (thrombopoietin) is the key regulator of thrombopoiesis for platelet lineage. In both mice and humans, inactivation of this gene results in significant decrease in number of mature platelets produced by marrow progenitors. Transgenic mice with increased expression of this protein show progressive fibrosis and osteosclerosis of the bone marrow, as well as histological changes in kidneys, leading to mesangioproliferative glomerulopathy.

General description

TPO (thrombopoietin) is a hematopoietic cytokine expressed in multiple tissue types. It is predominantly produced by liver and kidney and acts as a ligand for the cell surface receptor c-mpl.

Other Notes

SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF

Physical form

Lyophilized from Sodium Citrate, pH 4.5.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
1108AF466

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Chronic thrombopoietin overexpression induces mesangioproliferative glomerulopathy in mice.
Shimoda HK
American Journal of Hematology (2007)
Transcriptional regulation of bone marrow thrombopoietin by platelet proteins.
McIntosh B and Kaushansky K
Experimental Hematology (2008)
Kuter, D.J., et al.
Cytokine Reference, 965-982 (2001)
Krasimira Rozenova et al.
Blood, 125(11), 1730-1738 (2015-02-01)
Hematopoietic stem cell (HSC) self-renewal and multilineage reconstitution are controlled by positive and negative signaling cues with perturbations leading to disease. Lnk is an essential signaling adaptor protein that dampens signaling by the cytokine thrombopoietin (Tpo) to limit HSC expansion.

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