SRP3156
Sonic Hedgehog human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
HHG-1
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
0.8-1.0 μg/mL ED50
mol wt
20.0 kDa
packaging
pkg of 25 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... SHH(6469)
Related Categories
General description
The sonic hedgehog protein is encoded by the SHH gene that is mapped to human chromosome 7q36. It is homologous to the Drosophila segment polarity gene hedgehog. The encoded protein is a 45kDa precursor protein that is auto-cleaved to form SSH-N (an amino-terminal 20kDa product) and SSH-C (carboxy-terminal product). SSH-N is found to be cell-associated, while SSH-C diffuses freely. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring chemically modified Cys residue.
Application
Sonic Hedgehog human has been used:
- To study its role in liver cancer stem cell activity.
- For neuronal differentiation.
- For cholinergic-like neurons (ChLNs) differentiation.
The sonic hedgehog protein is used in neuronal induction medium as a supplement.
Biochem/physiol Actions
The SHH (sonic hedgehog) gene encodes a soluble signaling protein that functions in the patterning of cell differentiation in the neural tube and limb bud. It has also been implicated in several processes, such as developing nervous system, including midbrain and ventral forebrain neuronal differentiation and neuronal precursor proliferation. In adults, it regulates progenitor function and proliferation. Mutations in this gene have been associated with holoprosencephaly, a developmental defect of the forebrain and midface.
Sequence
IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Physical form
Lyophilized with no additives.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
监管及禁止进口产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The SHH/Gli axis regulates CD 90-mediated liver cancer stem cell function by activating the IL 6/JAK 2 pathway
Zhang K, et al.
Journal of Cellular and Molecular Medicine, 22(7), 3679-3690 (2018)
Direct transdifferentiation of human Wharton?s Jelly mesenchymal stromal cells into Cholinergic-like neurons
Mendivil-Perez M, et al.
Journal of Neuroscience Methods (2018)
Mutations in the human Sonic Hedgehog gene cause holoprosencephaly.
Roessler E
Nature Genetics, 14, 357-360 (1996)
Use of human induced pluripotent stem cell-derived neurons as a model for cerebral toxoplasmosis
Tanaka N, et al.
Microbes and Infection, 18(7-8), 496-504 (2016)
Induction of midbrain dopaminergic neurons by Sonic hedgehog.
Hynes M
Neuron, 15, 35-44 (1995)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service