SRP3067
IGF-Binding Protein-3 human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
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Synonym(s):
Growth-hormone-dependant binding protein, IBP-3
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.2 μg/mL
mol wt
28.8 kDa
packaging
pkg of 25 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IGFBP3(3486)
General description
Research area: Cell Signaling
IGFBPs (insulin-like growth factor-binding proteins) are mainly required to regulate IGFs (insulin like growth factors) activities. IGFBP3 is a multifunctional protein and is secreted by various cells. It is most ample circulating IGFBP and works in IGF-dependent and -independent mechanisms. The IGFBP3 gene is mapped to human chromosome 7p12.3.
Recombinant human IGF-BP3 is a 28.8kDa protein consisting of 264 amino acid residues.
IGFBPs (insulin-like growth factor-binding proteins) are mainly required to regulate IGFs (insulin like growth factors) activities. IGFBP3 is a multifunctional protein and is secreted by various cells. It is most ample circulating IGFBP and works in IGF-dependent and -independent mechanisms. The IGFBP3 gene is mapped to human chromosome 7p12.3.
Recombinant human IGF-BP3 is a 28.8kDa protein consisting of 264 amino acid residues.
Application
IGF-Binding Protein-3 human has been used to study its association with follicular fluid and subsequent effect on embryo development.
Biochem/physiol Actions
IGFBP3 (insulin-like growth factor-binding protein 3) is involved in cell survival as well as cell death and tumorigenesis. Epigenetic changes in IGFBP3 affect non-small-cell lung cancer, hepatocellular carcinoma, ovarian cancer, skin cancer, urological cancers, breast cancer and gastric cancer. IGFBP3 is a primary growth inhibitor and can reverse the mitogenic functions of IGF-1 (insulin like growth factor 1) by inducing apoptosis.IGFBP-3 functions both in an IGF-dependent and independent manner. Its IGF-dependent roles involve endocrine function by delivering IGFs to target cells and activating downstream signaling. Meanwhile, its IGF-independent roles include interactions with extracellular matrix and plasma membrane proteins, as well as translocation into the cytoplasm and nucleus. The C-terminal domain has cell-penetrating abilities, enabling the generation of short peptides for intracellular delivery of drugs or genes.
Sequence
GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK
Physical form
Lyophilized from 20mM HCl.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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The low IGFBP-3 level is associated with esophageal cancer patients: a meta-analysis.
Song G, et al.
World Journal of Surgical Oncology, 14, 307-307 (2016)
Shailly Varma Shrivastav et al.
Frontiers in cell and developmental biology, 8, 286-286 (2020-06-02)
Insulin-like growth factor (IGF) binding protein-3 (IGFBP-3), one of the six members of the IGFBP family, is a key protein in the IGF pathway. IGFBP-3 can function in an IGF-dependent as well as in an IGF-independent manner. The IGF-dependent roles
A genome-wide association study identifies novel loci associated with circulating IGF-I and IGFBP-3.
Kaplan RC, et al.
Human Molecular Genetics, 20, 1241-1241 (2011)
Insulin-like growth factor binding protein-3 mediates interleukin-24-induced apoptosis through inhibition of the mTOR pathway in prostate cancer.
Du Y, et al.
Oncology Reports, 34, 2273-2273 (2015)
Evaluation of Insulin-like Growth Factor-1 and Insulin-like Growth Factor Binding Protein-3 Expression Levels in Patients with Chronic Lymphocytic Leukemia.
Ayer M, et al.
Turkish journal of haematology : official journal of Turkish Society of Haematology, 33, 335-335 (2016)
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