SRP0415
PKM1 active human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Sign Into View Organizational & Contract Pricing
All Photos(2)
Synonym(s):
OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle
Recommended Products
biological source
human
recombinant
expressed in E. coli
Assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
59 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
1.8 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PKM(5315)
General description
Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.
Application
PKM1active human has been used in a cell-based kinase assay to study the ability ofa compound to inhibit the kinase activity of PKM1. It has also been used inoxidative stress experiments on PKM2.
Biochem/physiol Actions
Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
C Parkison et al.
Biochemical and biophysical research communications, 179(1), 668-674 (1991-08-30)
Using a T7 expression system, the monomer of rat pituitary pyruvate kinase, subtype M1 (PKM1), was overexpressed in Escherichia coli and purified to homogeneity. The monomeric p58-M1 has intrinsic enzymatic activity with a Vmax of 79 +/- 20 units/mg and
Research progress on the role of PKM2 in the immune response
Chunyan L, et al.
Frontiers in Immunology (2022)
Development of Novel Small-Molecule Activators of Pyruvate Kinase Muscle Isozyme 2, PKM2, to Reduce Photoreceptor Apoptosis.
Wubben, et al.
Pharmaceuticals (Basel, Switzerland), 16 (2023)
Evaluation of serum and pleural levels tumor M2-pyruvate kinase in lung cancer patients with pleural effusion
Tiantian Z, et al.
BMC Pulmonary Medicine, 22, 307-307 (2022)
Development of Novel Irreversible Pyruvate Kinase M2 Inhibitors
I-Shan H, et al.
Journal of Medicinal Chemistry, 62(18), 8497-8510 (2019)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service