SRP0339
Ceramide Kinase FLAG tag active human
recombinant, expressed in baculovirus infected Sf9 cells, ≥40% (SDS-PAGE)
Synonym(s):
CERK, LK4, hCERK
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
Assay
≥40% (SDS-PAGE)
form
aqueous solution
mol wt
60 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
0.4 mg/mL
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CERK(64781)
General description
Ceramide kinase (CERK) is a 537-amino acid protein. It has a catalytic region, a putative amino-myristoylation site in its amino terminal region and a pleckstrin homology domain. The gene encoding CERK is localized on human chromosome 22q13.31.
Human ceramide kinase (CERK) (GenBank Accession No. NM_022766), 1-537 (end) with C-terminal Flag-tag, MW= 60 kDa, expressed in Baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
Ceramide kinase (CERK) is an enzyme involved in sphingolipid metabolism and in the synthesis of ceramide-1-phosphate. It associates with calmodulin and at the time of Fc receptor-mediated phagocytosis, it aggregates in lipid rafts. CERK may have a role in calcium signaling. The protein functions in the phagocytosis of polymorphonuclear leukocytes and degranulation of mast cells.
Unit Definition
One unit is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Ceramide kinase: a novel lipid kinase molecular cloning and functional characterization
Sugiura M, et al.
The Journal of Biological Chemistry (2002)
A conserved cysteine motif essential for ceramide kinase function
Lidome E, et al.
Biochimie, 90(10), 1560-1565 (2008)
Tack-Joong Kim et al.
Biochemical and biophysical research communications, 342(2), 611-617 (2006-02-21)
Ceramide kinase (CERK) converts ceramide to ceramide-1-phosphate (C1P), which has recently emerged as a new bioactive molecule capable of regulating diverse cellular functions. The N-terminus of the CERK protein encompasses a sequence motif known as a pleckstrin homology (PH) domain.
Francesca Bini et al.
Neuropharmacology, 63(4), 524-537 (2012-05-15)
1α,25-Dihydroxyvitamin D3 (1,25(OH)₂D₃), a crucial regulator of calcium/phosphorus homeostasis, has important physiological effects on growth and differentiation in a variety of malignant and non-malignant cells. Synthetic structural hormone analogues, with lower hypercalcemic side effects, are currently under clinical investigation. Sphingolipids
Identification of genes associated with tumorigenesis of retinoblastoma by microarray analysis
Chakraborty S, et al.
Genomics, 90(3), 344-353 (2007)
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