SRP0283
Sphingosine kinase 1 Active human
recombinant, expressed in baculovirus, ≥80% (SDS-PAGE)
Synonym(s):
SK1, SPHK1, SPK 1
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
recombinant
expressed in baculovirus
Assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
46.9 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SPHK1(8877)
General description
Human Sphingosine kinase 1 (GenBank Accession No. AF200328), full length with N-terminal His tag, MW = 46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Unit Definition
One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Regulatory Information
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Sphingosine kinase 1 in viral infections
Carr, J., et al.
Reviews in Medical Virology, DOI: 10-DOI: 10 (2012)
Shi-Quan Liu et al.
International journal of colorectal disease, 27(12), 1569-1578 (2012-06-12)
Sphingosine kinase (SphK) 1 is an oncogenic enzyme promoting transformation, proliferation, and survival of a number of human tumor cells. However, its effect on colon cancer cell behavior has not been fully clarified. SphK1 plasmid or SphK1 shRNA transfection and
Deborah A Pasternack et al.
mBio, 6(5), e01291-e01215 (2015-10-08)
Sphingolipids are important constituents of cell membranes and also serve as mediators of cell signaling and cell recognition. Sphingolipid metabolites such as sphingosine-1-phosphate and ceramide regulate signaling cascades involved in cell proliferation and differentiation, autophagy, inflammation, and apoptosis. Little is
The key to the allergenicity of lipid transfer protein (LTP) ligands: A structural characterization.
Zulema Gonzalez-Klein et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1866(7), 158928-158928 (2021-03-28)
Plant lipid transfer proteins are a large family that can be found in all land plants. They have a hydrophobic cavity that allows them to harbor lipids and facilitates their traffic between membranes. However, in humans, this plant protein family
Jian Pan et al.
Journal of translational medicine, 9, 157-157 (2011-09-23)
Treatment failure for esophageal carcinoma is frequently due to lymph node metastasis and invasion to neighboring organs. The aim of the present study was to investigate invasion- and metastasis-related genes in esophageal carcinoma cells in vitro and in vivo. A
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