SRP0254
Cofilin 1 Active human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
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Synonym(s):
CFL1, Cofilin, non-muscle isoform, p18
UNSPSC Code:
12352202
NACRES:
NA.32
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biological source
human
recombinant
expressed in E. coli
Assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
44 kDa
packaging
pkg of 200 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CFL1(1072)
General description
Cofilin 1 is ubiquitously expressed and has a molecular mass of ~19kDa.
Human recombinant Cofilin1 (2-end) with N-terminal GST tag (GenBank Accession No. NM_005507), expressed in a E. coli expression system. Cofilin 1, MW = 44 kDa,
Human recombinant Cofilin1 (2-end) with N-terminal GST tag (GenBank Accession No. NM_005507), expressed in a E. coli expression system. Cofilin 1, MW = 44 kDa,
Application
Kinase substrate for the kinase assay; western blot
Biochem/physiol Actions
Cofilin-l (CFL1) plays a vital role in the regulation of actin cytoskeleton reorganization. It is also implicated in cancer cell migration and invasion in several types of tumor tissues. Elevated expression of the gene is associated with the development of esophageal squamous cell carcinoma (ESCC). In addition, abnormal expression of the gene leads to vulvar cancer and thus, it can be considered as a potential therapeutic target for the same. CFL1 facilitates cytokinesis, cell motility and morphogenesis. Activated CFL1 helps in preventing cytoskeletal-associated defects in axonal development stimulated by Syns.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Cofilin 1 activation prevents the defects in axon elongation and guidance induced by extracellular alpha-synuclein.
Tilve S
Scientific Reports (2015)
The role of cofilin-l in vulvar squamous cell carcinoma: A marker of carcinogenesis, progression and targeted therapy.
Wu Q
Oncology Reports, 35, 2743-2754 (2016)
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