SRP0249
PLK2 Active human
recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
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Polo-like kinase 2, SNK, Serum-inducible kinase
Recommended Products
biological source
human
recombinant
expressed in baculovirus infected insect cells
Assay
≥60% (SDS-PAGE)
form
aqueous solution
specific activity
≥3.6 pmol/min-μg
mol wt
calculated mol wt 107 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PLK2(10769)
General description
Human Plk2 (GenBank Accession No. NM_006622), full length, with N-terminal GST tag, expressed in a baculovirus infected Sf9 cell expression system. The theoretical MW is 107 kDa.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
新产品
Certificates of Analysis (COA)
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Bioorganic & medicinal chemistry, 24(4), 521-544 (2016-01-15)
Several families of protein kinases have been shown to play a critical role in the regulation of cell cycle progression, particularly progression through mitosis. These kinase families include the Aurora kinases, the Mps1 gene product and the Polo Like family
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