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Safety Information

SRP0247

Sigma-Aldrich

MAP3K14(NIK) Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym(s):

MAP3K14, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, NIK

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

88 kDa

packaging

pkg of 10 μg

concentration

>0.02 mg/mL

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... MAP3K14(9020)

General description

Human MAP3K14 (mitogen-activated protein kinase kinase kinase 14 ; NIK ; GenBank Accession No. NM_003954), (amino acids 318-end) with N-terminal GST tag, MW = 88 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Regulatory Information

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