SRP0219
CD45 Active human
recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)
Synonym(s):
CD45R, Leukocyte common antigen (LCA), PTPRC, Protein tyrosine phosphatase, receptor type, C, T200 leukocyte common antigen
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in baculovirus infected insect cells
Assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
75 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PTPRC(5788)
General description
Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 is expressed on T-cells and is found in multiple isoforms. The extracellular domain of the protein is predicted to have an amino-terminal mucin-like segment, a cysteine-rich domain and three fibronectin type 3 domains. The gene encoding this protein is localized on human chromosome 1q31.3.
Protein tyrosine phosphatase. Catalytic domain, (584-1256) (Gene bank accession number NM_080922) with N-terminal His tag, MW = 75 kDa, expressed in a Baculovirus Sf9 expression system.
Application
Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
Biochem/physiol Actions
Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 acts as a protein tyrosine phosphatase in leucocyte signaling. It has a role in flow cytometric diagnostics in leukemia. By dephosphorylation of Src kinases, the protein acts as a regulator of antigen receptor signaling in T and B cells. As a negative regulator of Janus kinases, CD45 inhibits cytokine receptor signaling.
Physical form
Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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CD45RO+ Memory T Lymphocytes ? a Candidate Marker for TNM-Immunoscore in Squamous Non?Small Cell Lung Cancer1
Erna-Elise Paulsen
Neoplasia, 17(11), 839-848 (2015)
Xinqi Wu et al.
Cancer immunology research, 5(6), 446-454 (2017-05-06)
The combination of anti-VEGF blockade (bevacizumab) with immune checkpoint anti-CTLA-4 blockade (ipilimumab) in a phase I study showed tumor endothelial activation and immune cell infiltration that were associated with favorable clinical outcomes in patients with metastatic melanoma. To identify potential
Initiation of T cell signaling by CD45 segregation at `close-contacts?
Veronica T Chang
Nature Immunology, 17(5), 574-582 (2016)
High CD45 surface expression determines relapse risk in children with precursor B-cell and T-cell acute lymphoblastic leukemia treated according to the ALL-BFM 2000 protocol
Gunnar Cario
Haematologica, 99(1), 103-110 (2014)
Impaired Glycoprotein VI-Mediated Signaling and Platelet Functional Responses in CD45 Knockout Mice.
Vaishali V Inamdar et al.
Thrombosis and haemostasis, 119(8), 1321-1331 (2019-06-22)
CD45 is a receptor protein tyrosine phosphatase present on the surface of all hematopoietic cells except for erythrocytes and platelets. Proteomics studies, however, have demonstrated the presence of a CD45 c-terminal catalytic peptide in platelets. Therefore, we investigated the functional
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