SRP0215
PTP1B full length Active human
recombinant, expressed in E. coli, aqueous solution, ≥55% (SDS-PAGE)
Synonym(s):
PTPN1, Protein tyrosine phosphatase, non-receptor type 1
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in E. coli
Assay
≥55% (SDS-PAGE)
form
aqueous solution
mol wt
76 kDa
packaging
pkg of 20 μg
storage condition
(Tightly closed)
avoid repeated freeze/thaw cycles
concentration
1.65 mg/mL
technique(s)
inhibition assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PTPN1(5770)
General description
Research area: Cell signaling
The gene PTPN1 (protein tyrosine phosphatase-1B) is mapped to human chromosome 20q13.13 and encodes a member of the protein tyrosine phosphatase (PTP) family. The open reading frame of 1305 bp encodes a protein of 435 amino acids. It is localized at the cytoplasmic side of the endoplasmic reticulum. PTP1B is characterized by a phosphatase catalytic domain and a proline-rich domain.
The gene PTPN1 (protein tyrosine phosphatase-1B) is mapped to human chromosome 20q13.13 and encodes a member of the protein tyrosine phosphatase (PTP) family. The open reading frame of 1305 bp encodes a protein of 435 amino acids. It is localized at the cytoplasmic side of the endoplasmic reticulum. PTP1B is characterized by a phosphatase catalytic domain and a proline-rich domain.
Application
PTP1B full length Active human is useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
PTPN1 has been used for dephosphorylation of PP2A (protein phosphatase 2A).It has also been used in recombinant protein tyrosine phosphatase-1B (PTP1B) assay.
Biochem/physiol Actions
The protein protein tyrosine phosphatase-1B (PTP1B) catalyzes the dephosphorylation of tyrosine residues of the activated insulin receptor and the insulin receptor substrate 1, thereby downregulating the insulin signaling cascade. It dephosphorylates JAK2 and STAT3 and inhibits leptin signaling. Polymorphism of this gene in mice has shown to be associated with type 2 diabetes and obesity. PTP1B dephosphorylates the epidermal growth factor receptor. It acts as a modulator of insulin-like growth factor receptor (IGFR) and MET-mediated signaling responses. The protein may be useful as a drug target in type 2 diabetes and obesity. It has been shown to enhance tumor progression in various cancers like that of the breast, colon and prostate. It acts a tumor suppressor in other cancers (lymphomas and esophageal cancers). PTP1B is implicated in the positive regulation of integrin and cadherin signaling.(1) In addition, human PTP1B is also involved in casein kinase II (CKII) and p60c-src-induced signal transduction pathway.
Unit Definition
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
antibody
Product No.
Description
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inhibitor
Product No.
Description
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related product
Product No.
Description
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WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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E J Jung et al.
Biochemical and biophysical research communications, 246(1), 238-242 (1998-05-26)
We have cloned a soluble chicken protein tyrosine phosphatase, named CPTP1, from the cDNA library of chicken intestine. The CPTP1 showed 92% sequence identity to the corresponding 321 amino acid residues of human PTP1B (HPTP1B). CPTP1 lacked 13 amino acids
PTP1B-dependent regulation of receptor tyrosine kinase signaling by the actin-binding protein Mena.
Hughes SK
Molecular Biology of the Cell, 26(21), 3867-3878 (2015)
Protein tyrosine phosphatase 1B (PTP1B): A key regulator and therapeutic target in liver diseases.
Chen PJ
Toxicology, 337, 10-20 (2015)
Alicja Kuban-Jankowska et al.
Antioxidants (Basel, Switzerland), 9(12) (2020-12-04)
The catechins derived from green tea possess antioxidant activity and may have a potentially anticancer effect. PTP1B is tyrosine phosphatase that is oxidative stress regulated and is involved with prooncogenic pathways leading to the formation of a.o. breast cancer. Here
Single nucleotide polymorphisms in protein tyrosine phosphatase 1beta (PTPN1) are associated with essential hypertension and obesity.
Olivier M
Human Molecular Genetics, 13, 1885-1892 (2004)
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