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SRP0195

Sigma-Aldrich

PARP6 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym(s):

MGC131971, Poly (ADP-ribose) Polymerase 6, pART17

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

98 kDa

packaging

pkg of 10 μg

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

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F3917D3658F6886
毛喉素 For use in molecular biology applications

F3917

毛喉素

毛喉素 from Coleus forskohlii, ≥98% (HPLC), powder

F6886

毛喉素

form

powder

form

powder

form

solid

form

powder

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

storage temp.

-

solubility

DMF:H2O (9:1): 2 mg/mL, clear

solubility

ethanol: soluble

solubility

methanol: 28 mg/mL(lit.), DMSO: 3 mg/mL(lit.), chloroform: 50 mg/mL, ethanol: 6.6 mg/mL(lit.), dilute aqueous acid and base: insoluble(lit.)

solubility

ethanol: soluble 50 mg/mL

color

white to beige

color

white to off-white

color

white to off-white

color

white to off-white

General description

PARP6 (poly(ADP-ribose) polymerase family member 6) gene is localized to human chromosome 15q23. It is a member of the family of multifunctional nuclear proteases known as PARPs, which contains 17 members and is further classified into three subtypes dependent on the catalytic domain structure. PARP6 is a type of mono(ADP-ribosyl) transferase on the basis that its catalytic domain has a significantly shorter nicotinamide-ribose-binding site than PARP1. This subfamily contains nine members including PARP6. PARP6 is composed of 630 residues and has a molecular weight of 71kDa.[1][2]
Human PARP6 (GenBank Accession No. NM_020214), full length with N-terminal GST tag, MW = 98kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

PARP6 (poly(ADP-ribose) polymerase family member 6) negatively controls cell cycle, as its ectopic expression in HeLa cells leads to suppression of cell growth. It functions as a tumor suppressor in CRC (colorectal cancer), and its expression is associated with good prognosis. The ectopic expression of PARP6 results in suppressed expression of the oncogene survivin. In CRC PAPR6 prevents colony formation, invasion, migration, and cell proliferation.[1][2]

Unit Definition

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Regulatory Information

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    Guangying Qi et al.
    Oncotarget, 7(14), 18812-18824 (2016-03-05)
    Poly (ADP-ribose) polymerases (PARPs) are enzymes that transfer ADP-ribose groups to target proteins and are involved in a variety of biological processes. PARP6 is a novel member, and our previous findings suggest that PARP6 may act as a tumor suppressor
    Handan Tuncel et al.
    International journal of oncology, 41(6), 2079-2086 (2012-10-09)
    Poly(ADP-ribose) polymerase (PARP) is an enzyme that mediates post-translational modification of proteins. Seventeen known members of the PARP superfamily can

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