SRP0187
Bcl-xL Active human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
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Synonym(s):
Apoptosis regulator Bcl-X, BCL-XL/S, BCL2L1, Bcl-2-like protein 1
UNSPSC Code:
12352200
NACRES:
NA.32
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biological source
human
recombinant
expressed in E. coli
Assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
28 kDa
packaging
pkg of 100 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... BCL2L1(598)
General description
BCL2L1 (B-cell lymphoma 2 like 1) gene codes for the anti-apoptotic protein called Bcl-xL (B-cell lymphoma-extra large) and its isoform Bcl-xS. Bcl-xS functions as an antagonist of Bcl-xL. This gene is localized to human chromosome 20q11.21.
Application
Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
BCL2L1 (B-cell lymphoma 2 like 1) or Bcl-xS functions as a dominant negative regulator of the anti-apoptotic isoform Bcl-xL. Bcl-xL is involved in modulating anti-apoptotic capacity of chemotherapy-resistant tumors. This protein is highly expressed in michigan cancer foundation 7 (MCF7) human breast carcinoma cells, and studies show that overexpression of Bcl-xS in these MCF-7 cells results in heightened sensitivity to chemotherapy-induced apoptosis by agents such as VP-16 or taxol.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Regulatory Information
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Certificates of Analysis (COA)
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Gain of 20q11.21 in human embryonic stem cells improves cell survival by increased expression of Bcl-xL.
Nguyen HT, et al.
Molecular Human Reproduction, 20(2), 168-177 (2014)
Bcl-xL mediates therapeutic resistance of a mesenchymal breast cancer cell subpopulation.
Keitel U, et al.
Oncotarget, 5(23), 11778-11791 (2014)
BCL-XL mediates the strong selective advantage of a 20q11.21 amplification commonly found in human embryonic stem cell cultures.
Avery S, et al.
Stem Cell Reports, 1(5), 379-386 (2013)
Francis McCoy et al.
Methods in molecular biology (Clifton, N.J.), 1133, 119-140 (2014-02-26)
The study of apoptosis and caspases has advanced greatly over recent decades. Studies conducted in the Xenopus laevis egg extract and oocyte model system have significantly contributed to these advances. Twenty years ago, Newmeyer and colleagues first showed that the
Overexpression of Bcl-XS sensitizes MCF-7 cells to chemotherapy-induced apoptosis.
Sumantran VN, et al.
Cancer Research, 55(12), 2507-2510 (1995)
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