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Safety Information

SRP0154

Sigma-Aldrich

SUV39H1 human

recombinant, expressed in E. coli, ≥50% (SDS-PAGE)

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Synonym(s):
Histone-lysine N-methyltransferase, H3 lysine-9 specific 1, KMT1A, Lysine N-methyltransferase 1A, Su(var)3-9 homolog 1, Suppressor of variegation 3-9 homolog 1
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥50% (SDS-PAGE)

form

aqueous solution

mol wt

64 kDa

packaging

pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SUV39H1(6839)

General description

Human SUV39H1, GenBank Accession No. NM_003173, amino acids 82-412, with N-terminal GST tag, MW = 64 kDa, expressed in E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Regulatory Information

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