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SRP0151

Sigma-Aldrich

SMYD2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym(s):

KMT3C, Lysine N-methyltransferase 3C, SET and MYND domain containing 2, ZMYND14, Zinc finger, MYND domain containing 14

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

49.7 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_020197

UniProt accession no.

Q9NRG4

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SMYD2(56950)

General description

SET and MYND domain containing 2 (SMYD2) is encoded by the gene mapped to human chromosome 1q32.3-q41. The encoded protein belongs to SMYD family of protein lysine methyltransferases.
Human SMYD2 (GenBank Accession No. NM_020197), full length with N-terminal DDDDK tag (FLAG), MW = 49.7kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

SET and MYND domain containing 2 (SMYD2) might act as a putative oncogene by methylating p53 and reducing its tumor suppressive role. Overexpression of SMYD2 gene is associated with pathogenesis and metastasis of oesophageal cancer, squamous cell carcinoma (ESCC). Therefore, it is considered as a potent therapeutic target for ESCC. SMYD2 has capacity to catalyze the methylation of histone and non-histone substrates. AZ505 acts as a potent and selective inhibitor of SMYD2 and aids in the improvement of novel cancer treatment.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

WGK

WGK 2

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Repression of p53 activity by Smyd2-mediated methylation.
Huang J
Nature, 444(7119), 629-632 (2006)
Makoto Nakakido et al.
Neoplasia (New York, N.Y.), 17(4), 367-373 (2015-05-01)
Phosphatase and tensin homologue (PTEN), one of the well-characterized tumor suppressor proteins, counteracts the phosphatidylinositol 3-kinase-AKT pathway through its unique lipid phosphatase activity. The functions of PTEN are regulated by a variety of posttranslational modifications such as acetylation, oxidation, ubiquitylation
Prediction of CCND1 amplification using plasma DNA as a prognostic marker in oesophageal squamous cell carcinoma.
Takeshita H
British Journal of Cancer, 102(9), 1378-1383 (2010)
Overexpression of SMYD2 relates to tumor cell proliferation and malignant outcome of esophageal squamous cell carcinoma.
Komatsu S
Carcinogenesis, 30(7), 1139-1146 (2009)
Structural basis of substrate methylation and inhibition of SMYD2.
Ferguson AD
Structure, 19(9), 1262-1273 (2011)

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